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pCk4_spacer

Plasmid

#136074

Purpose

Use when making even level constructs (L2,...) if pCk4 position (position 4) has no construct assigned

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pH6HTC_minus_cer-region

Plasmid

#175297

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Publication

Atanasoai et al bioRxiv 2021.11.08.467743

Type

Empty backbone

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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p40phox-PX (1-144)

Plasmid

#119123

Purpose

Bacterial expression of human phox homology (PX) domain, p40phox-PX (1-144)

Depositor

Brett Collins

Publication

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

p40phox-PX (1-144)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTYF-super-mfPENK-0.9k-Venus-WPRE

Plasmid

#156401

Purpose

Expresses Venus under PENK gene promoter

Depositor

Shuji Kaneko

Publication

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-super-mfPV-0.8k-Venus-WPRE

Plasmid

#156399

Purpose

Expresses Venus under PV gene promoter

Depositor

Shuji Kaneko

Publication

Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

Inserts

Venus
Gal4p65

Use

Lentiviral

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pUPD3

Plasmid

#118043

Purpose

GoldenBraid2.0 Universal Part Domesticator #3

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Domestication of dna parts for…

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOGG042

Plasmid

#113998

Purpose

Vector construction module. Tetracycline resistance module for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Tetracycline

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBSV2G_PflaB-msfCFPBb

Plasmid

#118232

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfCFP

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfCFP

Expression

Bacterial

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGDP4 ermC

Plasmid

#112884

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

Depositor

Gerard Wright

Publication

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

ermC (ermM Staphylococcus epidermis)

Tags

6xHis

Expression

Bacterial

Promoter

Plac

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pACYC.Taq.1Amb

Plasmid

#102794

Purpose

Expresses Taq DNA polymerases with a premature stop codon

Depositor

Andrew Ellington

Publication

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Expression

Bacterial

Mutation

W168Stop; *(see below)

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS268

Plasmid

#73726

Purpose

SapTrap SL2 C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

SL2 (gdp-2/gdp-3 intergenic region)

Use

CRISPR

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-hPDIb′a′

Plasmid

#60556

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Publication

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Tags

His6-hPDIb'a' tag

Expression

Bacterial

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pJY019

Plasmid

#32999

Depositor

James Fleckenstein

Publication

Fleckenstein et al Infect Immun. 2006 Apr . 74(4):2245-58.

Insert

etpBAC locus

Use

N/a

Tags

myc-6His

Expression

Bacterial

Promoter

pBAD

Available since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pGMCZ-PrpoB

Plasmid

#27073

Depositor

Dirk Schnappinger

Publication

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P-rpoB

Expression

Bacterial

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pKSJ155

Plasmid

#27126

Depositor

Alan Finkelstein

Publication

Kienker et al J Gen Physiol. 2000 Oct . 116(4):587-98.

Insert

Colicin Ia + Ia immunity protein

Tags

His6

Expression

Bacterial

Available since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-JEDI3sub-Kv-WPRE

Plasmid

#246618

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI3sub in AAV production vector for neuron -specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Publication

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3sub-Kv

Use

AAV

Tags

Kv

Expression

Mammalian

Promoter

hSyn

Available since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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pFast-hHus1

Plasmid

#222469

Purpose

Expresses hHus1 in insect cells

Depositor

Aziz Sancar

Publication

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hHus1 (HUS1 Human)

Expression

Insect

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLX311_CdTag-HA_EmptyVector

Plasmid

#221468

Purpose

Gateway backbone for C-terminal dTag cloning

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTP924 pHAGE2 CMV GFP

Plasmid

#199392

Purpose

Lentiviral transfer plasmid for constitutive expression of a GFP in mammalian cells

Depositor

Rebecca Voorhees

Publication

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

Plasmid

#199356

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Publication

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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