We narrowed to 32,661 results for: Eng

1. TRUPATH Triple GaZ

   Plasmid

   #196053

   Purpose

   Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaz-RLuc8 (GNAZ Human)
   GGamma1-GFP2 (GNGT1 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRUPATH Triple Gagust

   Plasmid

   #196054

   Purpose

   Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaGustducin-RLuc8 (GNAT3 Human)
   GGamma1-GFP2 (GNGT1 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVRC8400-SARS-CoV-2-S2P-XBB.1.5-AVI

   Plasmid

   #198465

   Purpose

   Expresses SARS-CoV-2 S2P protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-XBB.1.5 (S )
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   T19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

   Plasmid

   #60224

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   KrasG12D HDR donor (Kras Mouse)
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Promoter

   EFS, No promoter, and U6

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEB-C5

   Plasmid

   #28213

   Purpose

   5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit

   Depositor

   Linzhao Cheng

   Article

   Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

   Insert

   Oct4, Sox2, Klf4, c-Myc, Lin28 (Myc, Sox2, Pou5f1, Klf4, Lin28a Mouse)
   

   Expression

   Mammalian

   Available Since

   June 20, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. DH10B E. coli aptI/gidB::Landing Pad

   Bacterial Strain

   #83036

   Purpose

   For site-specific chromosomal insertion of a target DNA sequence at an engineered landing pad site in the atpI/gidB locus.

   Depositor

   Cammie Lesser

   Article

   Reeves et al ACS Synth Biol. 2015 May 15;4(5):644-54.

   Bacterial Resistance

   Tetracycline

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pVSVdG-4BFP2

   Plasmid

   #64101

   Purpose

   Expresses mTagBFP2

   Depositor

   Ian Wickersham

   Insert

   mTagBFP2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-T7pol

   Plasmid

   #59926

   Purpose

   Expression vector for T7 RNA polymerase gene

   Depositor

   Ian Wickersham

   Insert

   T7 RNA polymerase
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. PermaPhos Kit

   Plasmid Kit

   #1000000226

   Purpose

   This kit is used for expressing recombinant proteins with site-specific, non-hydrolyzable phosphoserine (nhpSer) in an engineered self-sufficient E. coli genetic code expansion (GCE) expression system

   Depositor

   Ryan Mehl

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

10. pRVdG-4BFP2

    Plasmid

    #52490

    Purpose

    Expresses mTagBFP2

    Depositor

    Ian Wickersham

    Insert

    mTagBFP2
    

    Use

    Rabies virus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRVdG-4GCaMP6s

    Plasmid

    #52497

    Purpose

    Expresses GCaMP6s

    Depositor

    Ian Wickersham

    Insert

    GCaMP6s
    

    Use

    Rabies virus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Denv2_GU289914.1_NS2B

    Plasmid

    #234401

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    NS2B_DENV2
    

    Tags

    MGSSHHHHHHSSGLVPRGS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Denv4_KJ160504.1_NS2B

    Plasmid

    #234402

    Purpose

    Bacterial expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    NS2B_DENV4
    

    Tags

    MGSSHHHHHHSSGLVPRGS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. NT-CRISPR plasmid collection

    Plasmid Kit

    #1000000189

    Purpose

    The NT-CRISPR plasmid collection contains all plasmids to perform genome engineering in Vibrio natriegens using the NT-CRISPR method.

    Depositor

    Anke Becker

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

15. pCAG-B19N

    Plasmid

    #59924

    Purpose

    Expression vector for Rabies virus SAD B19 nucleoprotein gene

    Depositor

    Ian Wickersham

    Insert

    Rabies virus nucleoprotein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-B19P

    Plasmid

    #59925

    Purpose

    Expression vector for Rabies virus SAD B19 phosphoprotein gene

    Depositor

    Ian Wickersham

    Insert

    Rabies virus phosphoprotein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-B19G

    Plasmid

    #59921

    Purpose

    Expression vector for Rabies virus SAD B19 glycoprotein gene

    Depositor

    Ian Wickersham

    Insert

    Rabies virus glycoprotein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOpen-Bstpol

    Plasmid

    #165543

    Purpose

    Full length DNA polymerase from Bacillus stearothermophilus.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    Bst DNA Polymerase, Full Length
    

    Use

    Synthetic Biology

    Available Since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pAAV-CAG-FLEX-splitTVA800

    Plasmid

    #59330

    Purpose

    Expresses splitTVA800

    Depositor

    Ian Wickersham

    Insert

    splitTVA800
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-FLEX-splitTVA950

    Plasmid

    #59329

    Purpose

    Expresses splitTVA950

    Depositor

    Ian Wickersham

    Insert

    splitTVA950
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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