We narrowed to 15,981 results for: LEA

1. pCMV_UdgX-EE-UdgX-nCas9-RBMX

   Plasmid

   #163565

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-nCas9-RBMX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-DIO-GRAB_g5-HT2m

   Plasmid

   #208722

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT2m in neurons in the presence of Cre recombinase

   Depositor

   Yulong Li

   Article

   Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT2m
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-B-HypaSpCas9-NLS-6xHis

   Plasmid

   #207386

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HypaSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A, amino acids 1005-1013…

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRESneo-FLAG/HA Ago2

   Plasmid

   #10821

   Depositor

   Thomas Tuschl

   Article

   Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

   Insert

   Argonaute 2 (AGO2 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   R668H. Additionally, N terminus different from wi…

   Available Since

   Oct. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTER E2F2shRNA human

   Plasmid

   #66884

   Purpose

   Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F2

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F transcription factor 2 (E2F2 Human)
   

   Use

   RNAi

   Expression

   Mammalian

   Promoter

   H1 Tet

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pBiex PKC - Peptide 8 mCit mCer

   Plasmid

   #83563

   Purpose

   Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

   Insert

   PRKCA (PRKCA Human)
   

   Tags

   10 nm ER/K Helix, FLAG purification tag, TEV Prot…

   Expression

   Bacterial and Insect

   Mutation

   Only catalytic domain of PKC (aa 335-672)

   Promoter

   T7 Promoter

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. HOXD4 (murine) MSCV

   Plasmid

   #8549

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXD4 (Hoxd4 Mouse)
   

   Use

   Retroviral

   Tags

   His and T7

   Expression

   Mammalian

   Mutation

   contains T7 and HIS tags from pET; makes protein …

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

   Plasmid

   #124660

   Purpose

   reporter protein which emits bright blue fluorescence

   Depositor

   Yutaka Kuroda

   Article

   Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

   Insert

   Gaussia luciferase-C9D-TGinsert
   

   Tags

   IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

   Expression

   Bacterial

   Mutation

   changed glutamic acid 100 to alanine, and glycine…

   Promoter

   T7 promoter

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Lyn-sACt-mCherry

   Plasmid

   #138216

   Purpose

   Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   Lyn-sACt-mCherry (Adcy10 Rat)
   

   Tags

   N-terminal targeting sequence from Lyn kinase and…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ165

    Plasmid

    #109327

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoter

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Insert

    zCas9
    

    Use

    CRISPR; Gateway compatible zcas9 entry clone

    Tags

    3x FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    Promoter EC1.2 enhancer fused to EC1.1 promoter

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-APPL1-ΔPTB

    Plasmid

    #59768

    Purpose

    Expresses APPL1 with PTB domain deleted

    Depositor

    Donna Webb

    Article

    Broussard et al Mol Biol Cell. 2012 Apr;23(8):1486-99. d

    Insert

    APPL1 (APPL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 467-709

    Promoter

    CMV

    Available Since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pCin4-3xFLAG-MKL1 S454A

    Plasmid

    #19844

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 S454A (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 454 to Alanine

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pMT-ubb-cytoBirA-2a-mCherry

    Plasmid

    #79885

    Purpose

    Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Ubiquitin

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRevTRE MKL1 S449A/T450A/S454A

    Plasmid

    #19847

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 S449A/T450A/S454A (MRTFA Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Mutation

    changed Serine 449, Threonine 450 and Serine 454 …

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pL90-Nat-2xHO

    Plasmid

    #139069

    Purpose

    CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

    Depositor

    Gianni Liti

    Article

    Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

    Insert

    Two guide RNAs targeting the HO endonuclease
    

    Expression

    Bacterial and Yeast

    Promoter

    TDH3

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pColdI-GST-mGTSF1l

    Plasmid

    #214817

    Purpose

    Bacterial expression of GST-tagged mouse GTSF1l

    Depositor

    Phillip Zamore

    Article

    Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

    Insert

    GTSF1l (Gtsf1l Mouse)
    

    Tags

    6x His and GST

    Expression

    Bacterial

    Promoter

    CspA

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT-MCS-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80058

    Purpose

    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Tags

    3x HA

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_EE-NG-nCas9

    Plasmid

    #163542

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    EE-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75232

    Purpose

    CRISPR/Cas9 plasmid against human NFATc2

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc2 (NFATC2 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. DICER KO vector

    Plasmid

    #16656

    Depositor

    Bert Vogelstein

    Article

    Cummins et al Proc Natl Acad Sci U S A. 2006 Mar 7. 10

    Insert

    Dicer knock-out (DICER1 Human)
    

    Use

    Adeno-associated viral

    Expression

    Mammalian

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only