We narrowed to 9,277 results for: Pol;

1. mOC-STAMP (N162D)_3xFlag-Myc-CMV26

   Plasmid

   #73578

   Purpose

   Express mouse OC-STAMP ((N162D) in mammalian cells

   Depositor

   Paul Odgren

   Article

   Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

   Insert

   OC-STAMP (Ocstamp Mouse)
   

   Tags

   3x Flag and c-Myc tag

   Expression

   Mammalian

   Mutation

   changed asparagin (N) 162 to Aspartic Acid (D)

   Available Since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pGEX-GST-CHD5-WT-FRAG

   Plasmid

   #68873

   Purpose

   IPTG inducible bacterial expression of CHD5 fragment

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5 (CHD5 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   331-672aa

   Promoter

   tac

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCR2.1-Ins2-842

   Plasmid

   #53969

   Purpose

   842 bp insert from mouse Ins2 gene used as a control for methylation-specific PCR assays

   Depositor

   Kevin Ferreri

   Article

   Husseiny et al PLoS One. 2012;7(10):e47942. doi: 10.137

   Insert

   mouse Ins2 gene partial (Ins2 Mouse)
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe-GFP-IKBalpha-mut (super repressor)

   Plasmid

   #15264

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKB alpha (NFKBIA Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Serine 32 to Alanine, Serine 36 to Alanine

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-10-3xFLAG-LGP2-K651E

   Plasmid

   #58685

   Purpose

   Expresses LGP2 with a mutation in the CTD (K651E) in mammalian cells

   Depositor

   Curt Horvath

   Article

   Bruns et al J Biol Chem. 2013 Jan 11;288(2):938-46.

   Insert

   LGP2-K651E (DHX58 Human)
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   mutation to CTD, K651E

   Promoter

   CMV

   Available Since

   July 31, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB

   Plasmid

   #193717

   Purpose

   Rapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubules

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB (KIF5B Human, Synthetic)
   

   Tags

   2xmNeongreen-FRB

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB80-hsKIF5B(1-560)Rigor-L-mVenus

   Plasmid

   #193720

   Purpose

   Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)Rigor-L-mVenus (KIF5B Human, Synthetic)
   

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

   Plasmid

   #193719

   Purpose

   Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport

   Depositor

   Lukas Kapitein

   Article

   Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

   Insert

   hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Human, Synthetic)
   

   Tags

   2xHA-FRB

   Expression

   Mammalian

   Mutation

   hsKIF5B(aa1-560), RIGOR: G234A

   Promoter

   Chicken beta-actin

   Available Since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-BRCA1

   Plasmid

   #115491

   Purpose

   EGFP-BRCA1 expression vector. Parton clone BXY

   Depositor

   Rob Parton

   Article

   McMahon et al Elife. 2021 Jun 18;10. pii: 61407. doi:

   Insert

   BRCA1 (BRCA1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S1009R (See depositor comments)

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-Puro-IKBalpha-mut (super repressor)

    Plasmid

    #15291

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    inhibitor of Kappa B alpha (NFKBIA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    S32A/S36A: “super-repressor” allele (resistant to…

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid

    #208942

    Purpose

    The CE1 construct, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCJ136

    Plasmid

    #162665

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA528 CHMP1B 1-199

    Plasmid

    #115326

    Purpose

    Expresses residues 1-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-24

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX459v3

    Plasmid

    #178799

    Purpose

    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-N1 Full length Importin beta

    Plasmid

    #106941

    Purpose

    Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

    Depositor

    Patrizia Lavia

    Article

    Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

    Insert

    human Importin beta-1 (KPNB1) (KPNB1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEMHE-mScarlet-MAP4-MTBD

    Plasmid

    #171488

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged mouse MAP4-Microtubule Binding Domain mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    MAP4-MTBD (Map4 Mouse)
    

    Use

    Pgehme

    Tags

    mScarlet

    Mutation

    Aminoacids 658-1126

    Promoter

    T7

    Available Since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRosa26-EF1a-hCas9IRESneo

    Plasmid

    #67987

    Purpose

    Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-hCas9IRESneopA
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEMHE-miRFP-MAP4-MTBD

    Plasmid

    #171487

    Purpose

    T7 promotor drives in vitro transcription of miRFP-tagged mouse MAP4-Microtubule Binding Domain mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    MAP4-MTBD (Map4 Mouse)
    

    Use

    Pgehme

    Tags

    miRFP

    Mutation

    Aminoacids 658-1126

    Promoter

    T7

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX037

    Plasmid

    #167156

    Purpose

    MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    caffeic acid cycle genes
    

    Expression

    Plant

    Promoter

    pNOS, p35s

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRIPZ-bioGEF-UBwt-PURO

    Plasmid

    #208045

    Purpose

    Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    Ub (UBC Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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