We narrowed to 7,272 results for: tet

1. pMRE-Tn5-164

   Plasmid

   #118544

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mOrange2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRE-Tn5-166

   Plasmid

   #118546

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mCardinal
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDule-Acd82

   Plasmid

   #174099

   Purpose

   Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

   Depositor

   Ryan Mehl

   Article

   Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

   Insert

   Acridone 82 tRNA synthetase and tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N311S, C313G, V366A, W382T

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFAB1935

   Plasmid

   #47860

   Purpose

   BIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   his_T_min_linker_crp_T_min double terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   concatenate of his_min and crp_min

   Promoter

   pLTetO15

   Available since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9GG

   Plasmid

   #131009

   Purpose

   Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Inserts

   direct repeat of SpCas9
   promoter PJ23119
   mRFP expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_R0040 TetR

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJZC39

   Plasmid

   #62333

   Purpose

   sgRNA + 1x PP7 with mCherry for mammalian cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA + 1x PP7
   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

   Promoter

   CMV and U6

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCD5-ss-D/bovine/France/5920/2014-HEFs57aED-GCN4-sfGFP-ST

   Plasmid

   #175018

   Purpose

   Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

   Insert

   OK-HEF s57a ED
   

   Use

   Tags

   GCN4-TEV-sfGFP-TwinStrep

   Expression

   Mammalian

   Mutation

   s57a esterase knock out mutant

   Promoter

   CMV

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO/SH/GW-Mst1

   Plasmid

   #84299

   Purpose

   Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.

   Depositor

   Jonathan Chernoff

   Article

   Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71

   Insert

   Mst1 (STK4 Human)
   

   Use

   Tags

   HA, SBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pBT250_(pCA-G-intron(Neo)-tTA2)

   Plasmid

   #36876

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tTA2
   beta-globin intron
   Neo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available since

   Aug. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKF-P14MMP2AG-T2APuroR-5h

    Plasmid

    #128256

    Purpose

    Mammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    rtTA-Advanced::P2A::EGFP::T2A::PuroR
    

    Use

    Synthetic Biology; Flp-in expression vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. JPF0419v

    Plasmid

    #124021

    Purpose

    Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCAG-Lyn-iRFP713_pCAG-NES-mAzamiGreen_pCAG-p38KTR-mCerulean_pCAG-H2B::mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT3-RhoAC

    Plasmid

    #100032

    Purpose

    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with adenylyl cyclase activity.

    Depositor

    Daniel Oprian

    Article

    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

    Insert

    RhoAC
    

    Use

    Tags

    1D4 (TETSQVAPA)

    Expression

    Mammalian

    Mutation

    E497K/C566D

    Promoter

    Adenovirus major late promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEM-Cas9noSsrA

    Plasmid

    #102285

    Purpose

    Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    removed ssrA tag

    Promoter

    pTet

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT3-RhoGC E254D

    Plasmid

    #100033

    Purpose

    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.

    Depositor

    Daniel Oprian

    Article

    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

    Insert

    RhoGC E254D
    

    Use

    Tags

    1D4 (TETSQVAPA)

    Expression

    Mammalian

    Mutation

    E254D

    Promoter

    Adenovirus major late promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pFAB1902

    Plasmid

    #47858

    Purpose

    BIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    M13_central_T_linker_rrnD_T1 double terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    concatenate of M13 central and rrnD

    Promoter

    pLTetO13

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCIFR-5

    Plasmid

    #233294

    Purpose

    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    msfGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    p14G-BCD2

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFNC-5

    Plasmid

    #233297

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. TcR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Inserts

    BxbI phage integrase
    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PEM7 and unknown

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bxb1-GA_CAG-rtTA-T2A-mTagBFP2

    Plasmid

    #229797

    Purpose

    Bxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC-hU6-crBFP_EF1a-BFP

    Plasmid

    #224785

    Purpose

    BFP targeting crRNA for RfxCas13d expressed from hU6 promoter and target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC-hU6-CrEGFP_EF1a-BFP

    Plasmid

    #224786

    Purpose

    EGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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