We narrowed to 10,238 results for: ada

1. pCMV_RBMX-eA3A-UdgX-HF-NG-nCas9

   Plasmid

   #163550

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   RBMX-eA3A-UdgX-HF-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMKO.1-shCenpf

   Plasmid

   #160956

   Purpose

   Cenpf shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Cenpf shRNA (Cenpf Mouse)
   

   Use

   RNAi and Retroviral

   Promoter

   U6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. prCOrev

   Plasmid

   #153961

   Purpose

   Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
   

   Use

   Retroviral; A reporter plasmid detecting dna reco…

   Tags

   NLS-ZF

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   LTR

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. prCO

   Plasmid

   #153960

   Purpose

   Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
   

   Use

   Retroviral; A reporter plasmid detecting dna reco…

   Tags

   NLS-ZF

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   LTR

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. S2 1058 ABEMax

   Plasmid

   #135356

   Purpose

   Expression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-ABEMax aa 1058
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. S1 1029 CP ABEMax strategy 3

   Plasmid

   #135359

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   1029 CP nSpyCas9 ABEMax
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11A

   Plasmid

   #159131

   Purpose

   Expresses BACH1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

   Insert

   BACH1 (BACH1 Human)
   

   Tags

   2X-FLAG _ 2X-STREP

   Expression

   Mammalian

   Mutation

   Y11A

   Promoter

   CMV

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. p-AAV2-hSyn-MerMAID1-Citrine

   Plasmid

   #126520

   Purpose

   Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

   Depositor

   Peter Hegemann

   Article

   Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

   Insert

   MerMAID1
   

   Use

   AAV

   Tags

   Citrine

   Promoter

   human synapsin

   Available Since

   Nov. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AdMKP-3 S159AS197A

   Plasmid

   #131523

   Purpose

   Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells

   Depositor

   Haiyan Xu

   Article

   Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

   Insert

   Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
   

   Use

   Adenoviral

   Tags

   No

   Mutation

   changed Serine 159 to Alanine and Serine 197 to A…

   Promoter

   CMV

   Available Since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-INTS3-C

    Plasmid

    #128642

    Purpose

    Express INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    INTS3 C-termini (INTS3 Human)
    

    Tags

    His tag on N and C-terminal

    Expression

    Bacterial

    Mutation

    INTS3 C-termini (514–1042 aa)

    Promoter

    T7

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p-mCherry-C1-MerMAID2

    Plasmid

    #126514

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID2
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+ enhancer)

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p-mCherry-C1-MerMAID5

    Plasmid

    #126517

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID5
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+ enhancer)

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-mCherry-C1-MerMAID3

    Plasmid

    #126515

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID3
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+ enhancer)

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p-mCherry-C1-MerMAID6

    Plasmid

    #126518

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID6
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+ enhancer)

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-mCherry-C1-MerMAID4

    Plasmid

    #126516

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID4
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+ enhancer)

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. p-mCherry-C1-MerMAID7

    Plasmid

    #126519

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID7
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+ enhancer)

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. p-mCherry-C1-MerMAID1

    Plasmid

    #126513

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID1
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV (+ enhancer)

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX311 DEDD

    Plasmid

    #117745

    Purpose

    Open reading frame vector encoding DEDD

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    DEDD1 (DEDD Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKK-TEV-mCardinal

    Plasmid

    #105798

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCardinal

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKK-TEV-mCerulean

    Plasmid

    #105790

    Purpose

    Expression of your protein of interest in fusion with cyan fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCerulean

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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