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pAW8_13Myc

Plasmid

#110232

Purpose

Cre/Lox C-terminal tagging construct encoding 13 copies of the human c-myc (Myc) epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUCHR-mClover-smAID-P2A-CD5Flag2-bglpA

Plasmid

#110654

Purpose

Lentiviral vector expressing cell surface Flag-tag and monomeric GFP C-terminaly fused with auxin inducible degron

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

mClover-AID-P2A-CD5Flag2-bglpA

Use

Lentiviral

Promoter

CMV

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pUCHR-mClover-smAID-P2A-CD5cmyc2-bglpA

Plasmid

#110656

Purpose

Lentiviral vector expressing cell surface c-Myc-tag and monomeric GFP C-terminaly fused with auxin inducible degron

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

mClover-AID-P2A-CD5cmyc2-bglpA

Use

Lentiviral

Promoter

CMV

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pUCHR-CD5HA2-P2A-smAID-mClover

Plasmid

#110658

Purpose

Lentiviral vector expressing cell surface HA-tag and monomeric GFP N-terminaly fused with auxin inducible degron

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CD5HA2-P2A-AID-mClover

Use

Lentiviral

Promoter

CMV

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJet-CMV-CD5cmyc2-bglpA

Plasmid

#110645

Purpose

Expression plasmid for c-Myc-tag embedded into human CD52 with short polyA

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CMV-CDcmyc2-bglpA

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAW5

Plasmid

#110221

Purpose

Cre-expression plasmid

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAW1

Plasmid

#110220

Purpose

integration of loxP-ura4+-loxM3 cassette

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Insert

ura4+ (ura4 Fission Yeast)

Use

Cre/Lox

Mutation

See depositor comments below.

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW32

Plasmid

#110225

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt1 promoter

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW33

Plasmid

#110226

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt41 promoter

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW34

Plasmid

#110227

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt81 promoter

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW41

Plasmid

#110228

Purpose

integration of loxP site a distance upstream of gene start site

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Insert

ura4+ (ura4 Fission Yeast)

Use

Cre/Lox

Mutation

See depositor comments below.

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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px330 Gatad2a sgRNA (for deletion)

Plasmid

#110814

Purpose

Expressed sgRNA for generating Gatad2a deletion (knockout ) in mouse cell lines

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Expression

Mammalian

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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px330 Gatad2a sgRNA (for flox targeting)

Plasmid

#110815

Purpose

Used with pNTK Gatad2a flox allele targeting construct in mouse cells

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Expression

Mammalian

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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B. adolescentis mRNA toehold switch sensor

Plasmid

#110710

Purpose

Toehold switch sensor to detect a species specific mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. adolescentis species specific toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-Sfxn2 (CG6812)

Plasmid

#110643

Purpose

Retroviral expression of Flag-tagged Drosophila CG6812 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

CG6812 (CG6812 Fly)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-Sfxn1-3 (CG11739)

Plasmid

#110642

Purpose

Retroviral expression of Flag-tagged Drosophila CG11739 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

CG11739 (CG11739 Fly)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8

Plasmid

#110222

Purpose

Cre-expression plasmid for cassette exchange

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_yEGFP

Plasmid

#110229

Purpose

Cre/Lox C-terminal tagging construct encoding codon-optimized green fluorescent protein

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV500

Plasmid

#110722

Purpose

Lentiviral system for reduced ectopic expression.

Depositor

Kevin Dean, Reto Fiolka

Article

Dean et al Biophys J. 2016 Mar 29;110(6):1456-65. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAW8_1XFLAG

Plasmid

#110234

Purpose

Cre/Lox C-terminal tagging construct encoding single copy of the FLAG epitope

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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