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pDONR223_SAMHD1_WT

Plasmid

#81899

Purpose

Gateway Donor vector containing SAMHD1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SAMHD1 (SAMHD1 Human)

Use

Gateway Cloning

Mutation

G404E; A546V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CREB1_WT

Plasmid

#82203

Purpose

Gateway Donor vector containing CREB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CREB1 (CREB1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b-RFND

Plasmid

#195339

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis and 6xHis, MBP, TEV cleavage site

Expression

Bacterial

Mutation

R160E, F208W, N524V, D868V

Promoter

T7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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LentiRCas9-Lambda2

Plasmid

#104184

Purpose

Lentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

Depositor

Eugene Yeo

Article

Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

Insert

U6-sgRNA, EFS-PIN-dCas9

Use

CRISPR and Lentiviral

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT6_WT

Plasmid

#81950

Purpose

Gateway Donor vector containing STAT6, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT6 (STAT6 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CLOCK_WT

Plasmid

#82247

Purpose

Gateway Donor vector containing CLOCK , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CLOCK (CLOCK Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPARG_WT

Plasmid

#82223

Purpose

Gateway Donor vector containing PPARG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPARG (PPARG Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAPK1_WT

Plasmid

#82145

Purpose

Gateway Donor vector containing MAPK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAPK1 (MAPK1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH1_p.R132C

Plasmid

#81726

Purpose

Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH1 (IDH1 Human)

Use

Gateway Cloning

Mutation

R132C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARNTL_WT

Plasmid

#82189

Purpose

Gateway Donor vector containing ARNTL , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ARNTL (BMAL1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AXIN2_WT

Plasmid

#82099

Purpose

Gateway Donor vector containing AXIN2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AXIN2 (AXIN2 Human)

Use

Gateway Cloning

Mutation

P50S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEGBacmam-NGS Wnt-Fc

Plasmid

#159629

Purpose

Express Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid research

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7/8 subtype NGS Wnt Fc

Tags

Fc tag and His tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_WT

Plasmid

#81923

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TMPO_WT

Plasmid

#81818

Purpose

Gateway Donor vector containing TMPO , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TMPO (TMPO Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G12V

Plasmid

#81665

Purpose

Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

G12V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_EZH2_WT

Plasmid

#81742

Purpose

Gateway Donor vector containing EZH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EZH2 (EZH2 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only
pDEST-FRT/TO-3xFLAG-REV7

Plasmid

#114127

Purpose

Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

REV7 (MAD2L2 Human)

Use

Genomic integration, tetracycline inducible

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_GATA3_WT

Plasmid

#81902

Purpose

Gateway Donor vector containing GATA3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GATA3 (GATA3 Human)

Use

Gateway Cloning

Mutation

V3A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMARCB1_WT

Plasmid

#81791

Purpose

Gateway Donor vector containing SMARCB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMARCB1 (SMARCB1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH2_WT

Plasmid

#81800

Purpose

Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge