We narrowed to 10,563 results for: ESP

1. p426_Cas9_gRNA-ARS1622b

   Plasmid

   #87403

   Purpose

   All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1622b sequence GTCACGTTCCTGAGGTTACT in yeast chromosome 16.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1622b
   

   Use

   CRISPR

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-HA p21 K161R, K163R

   Plasmid

   #78789

   Purpose

   To overexpress p21 K161R, K163R in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

   Insert

   p21 (CDKN1A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K161R, K163R_muation

   Promoter

   CMV

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCin4-3xFLAG-MKL1 S449A/T450A/S454A

   Plasmid

   #19845

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 S449A/T450A/S454A (MRTFA Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Serine 449, Threonine 450 and Serine 454 …

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA5/BirA/FLAG-DDX54

   Plasmid

   #97063

   Purpose

   Expresses BirA/FLAG-tagged DDX54 in mammalian cells

   Depositor

   Markus Landthaler

   Article

   Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

   Insert

   DDX54 (DDX54 Human)
   

   Tags

   BirA and FLAG

   Expression

   Mammalian

   Promoter

   tetO2

   Available Since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST27-GST-ATXN1 FL(92Q)-S776D

   Plasmid

   #21757

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 92 Q(Glutamines) and…

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCS3-MT-BX-6MYC p21

   Plasmid

   #78780

   Purpose

   To overexpress p21 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

   Insert

   p21 (CDKN1A Human)
   

   Tags

   6xMYC

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pEGFP-RNF8-Middle-part

   Plasmid

   #64672

   Purpose

   Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   ring finger protein 8 (RNF8 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Containing only middle part of RNF8 amino acids 1…

   Promoter

   CMV

   Available Since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer

   Plasmid

   #176588

   Purpose

   Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associate IscB (Delaware Bay) locus
   

   Expression

   Bacterial

   Mutation

   Replaced spacer in CRISPR array with Fn spacer

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG71 BDNF 3'UTR mut

   Plasmid

   #12054

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Insert

   BDNF 3'UTR mut (BDNF Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutations in microRNA recognition sites (seed mat…

   Available Since

   June 2, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDN-T1TCbt

    Plasmid

    #44514

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-T123 promoter (GAL1 Synthetic, Budding Yeast)
    tetR::ymcherry
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Mutation

    Three tandem tet operators (3XtetO2) downstream o…

    Promoter

    PGal1-T123 promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDN-T1GZmbh

    Plasmid

    #44522

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-T123 promoter (GAL1 Synthetic, Budding Yeast)
    yEGFP::ZeoR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Mutation

    Three tandem tet operators (3XtetO2) downstream o…

    Promoter

    PGal1-T123 promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p426_Cas9_gRNA-ARS911b

    Plasmid

    #87394

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS911b sequence GTAATATTGTCTTGTTTCCC in yeast chromosome 9.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS911b
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pαH-S-HRV3C/D614G

    Plasmid

    #164568

    Purpose

    SARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-HRV3C-D614G variant)
    

    Tags

    2X Strep-Tag II and 8X His tag

    Expression

    Mammalian

    Mutation

    D614G

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_Hygro(+)_GRHL1-L3F6H

    Plasmid

    #177765

    Purpose

    Overexpression of 3xFLAG-6xHis tagged human GRHL1

    Depositor

    Michael Ristow

    Article

    Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

    Insert

    GRHL1 (GRHL1 Human)
    

    Expression

    Mammalian

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-N3/hArf5(WT)-EGFP

    Plasmid

    #79534

    Purpose

    Expresses C-terminally EGFP-tagged Arf5(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf5 (ARF5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDN-G1TCbt

    Plasmid

    #44515

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
    tetR::ymcherry
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available Since

    April 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQCXIB Flag MFF WT

    Plasmid

    #74384

    Purpose

    Retroviral expression vector containing Flag tagged WT MFF isoform 5

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pDN-G1GZmbh

    Plasmid

    #44516

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
    yEGFP::ZeoR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST27-GST-ATXN1 FL(30Q)-S776D

    Plasmid

    #21755

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 32 amino acids (30 Q…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

20. GST-SF3B2 wild type aa 401-550 fragment

    Plasmid

    #67615

    Purpose

    bacterial expression of wild type GST-SF3B2 fragment (401-550)

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    splicing factor 3b subunit 2 (SF3B2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    fragment of amino acids 401-550

    Promoter

    tac

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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