We narrowed to 10,185 results for: ADA

1. pAAV-hSyn-DIO-GRAB_CRF1.0

   Plasmid

   #208661

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 Mfn2-HA S27D

   Plasmid

   #139194

   Purpose

   Expresses C-terminal HA tagged human Mfn2 S27D in mammalian cells

   Depositor

   Allan Weissman

   Article

   Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

   Insert

   MFN2 (MFN2 Human)
   

   Tags

   2HA

   Expression

   Mammalian

   Mutation

   Serine 27 to Aspartic Acid

   Promoter

   CMV promoter

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCRY1 HA tag

   Plasmid

   #110298

   Purpose

   full length wt mouse Cry1 HA tag in pcDNA3.1

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

   Insert

   wt mcry1 HA tag (Cry1 Mouse)
   

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   wildtype

   Promoter

   CMV

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-cT3G-cERP2-YBX1

   Plasmid

   #192904

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of YBX1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   YBX1 (YBX1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Puro-moxGFP-LMNB1

   Plasmid

   #207773

   Purpose

   Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAAV-UBC-S1-GCaMP6f

   Plasmid

   #160722

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S1-GCaMP6f
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Puro-mNeon-ACTB

   Plasmid

   #207752

   Purpose

   Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLOC-MCOLN1

   Plasmid

   #131583

   Purpose

   For expression of human MCOLN1 in mammalian cells, GFP-tagged

   Depositor

   Sean Morrison

   Article

   Kasitinon et al Cell Rep. 2019 Aug 27;28(9):2293-2305.e9

   Insert

   MCOLN1 (MCOLN1 Human)
   

   Use

   Lentiviral

   Tags

   turboGFP tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKK-TEV-EGFP

   Plasmid

   #105793

   Purpose

   Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-EGFP

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPRv2_sgCtrl#1

    Plasmid

    #174148

    Purpose

    Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. NC14 pGLUE FBXW11

    Plasmid

    #36969

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    FBXW11 (FBXW11 Human)
    

    Tags

    CBP tag, HA, and streptag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pR26-CMVconst-mBMP7

    Plasmid

    #127375

    Purpose

    Allows for constitutive BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

    Depositor

    Lance Miller

    Article

    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

    Insert

    BMP7 (Bmp7 Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Promoter

    CMV enhancer/promoter

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Circular 200,100 (RAB7A)

    Plasmid

    #170118

    Purpose

    AAV vector carrying a guide RNA targeting the human RAB7A mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (RAB7A Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-C-Solo-mStayGold-COX8A

    Plasmid

    #227309

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    COX8A Homology Arms flanking a mStayGold Tag (COX8A Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40

    Plasmid

    #101061

    Purpose

    AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Has Service

    AAV1

    Insert

    CaMPARI2-F391W
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTwist-SARS-CoV-2 Spike-deltaC18 JN.1.11.1

    Plasmid

    #223272

    Purpose

    Encodes SARS-CoV-2 spike JN.1.11.1 for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike JN.1.11.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. CTCF-mAC donor (Neo)

    Plasmid

    #140645

    Purpose

    CTCF tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CTCF (CTCF Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. hU6-pegRNA-EF-1α-Cre Template

    Plasmid

    #196036

    Purpose

    The template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.

    Depositor

    Tyler Jacks

    Article

    Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hU6 and EF-1-alpha

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-M4-AR-S650D

    Plasmid

    #171246

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Asp (AR-S650D)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    3xFlag-tagged human androgen receptor Ser650Asp mutant (AR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Ser650Asp (AR-S650D)

    Promoter

    CMV

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Flag-M4-AR-S650A

    Plasmid

    #171245

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Ala (AR-S650A)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    3xFlag-tagged human androgen receptor Ser650Ala mutant (AR Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Ser650Ala (AR-S650A)

    Promoter

    CMV

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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