We narrowed to 7,272 results for: tet

1. pcDNA5-FRT-TO-FH-Nsp13

   Plasmid

   #157713

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp13 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pET28a(+) 6xHis-TEV-IFIT2

   Plasmid

   #53558

   Purpose

   bacterial expression of IFIT2

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT2 (IFIT2 Human)
   

   Use

   Tags

   6xHIS and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5-FRT-TO-Nsp12-HF

   Plasmid

   #157711

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp12-HF (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pcDNA5-FRT-TO-FH-Nsp2

   Plasmid

   #157683

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp2 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pHR-SUMO-A'-l4-G'-IRES-EmGFP

   Plasmid

   #209127

   Purpose

   Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G' and EmGFP

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   SUMO-A'-l4-G'-IRES-EmGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHR-SUMO-A'-l4-G-IRES-EmGFP

   Plasmid

   #209126

   Purpose

   Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G and EmGFP

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   SUMO-A'-l4-G-IRES-EmGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5-FRT-TO-Nsp8-HF

   Plasmid

   #157696

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp8-HF (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pINTO-NSA::hSNRNP70

   Plasmid

   #61726

   Purpose

   Expression of human SNRNP70 fused to monomeric streptavidin (N-terminus)

   Depositor

   Roberto Bonasio

   Article

   Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

   Insert

   SNRP70 (SNRNP70 Human)
   

   Use

   Tags

   mSA (monomeric streptavidin)

   Expression

   Mammalian

   Mutation

   Two silent mutations to ensure restriction site c…

   Promoter

   T-REx (CMV + 2xTetO2)

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJZC25

   Plasmid

   #62328

   Purpose

   sgRNA + 1x MS2 with MCP-VP64 effector for mammalian cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA + 1x MS2 binding module
   MCP-VP64
   

   Use

   Lentiviral

   Tags

   VP64

   Expression

   Mammalian

   Mutation

   Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

   Promoter

   CMV and U6

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-FRT-TO-FH-Nsp5

    Plasmid

    #157686

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp5 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pcDNA5-FRT-TO-Nsp7

    Plasmid

    #157688

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA5-FRT-TO-FH-Orf6

    Plasmid

    #157727

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Orf6 (ORF6 SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pB-TAG-HES7

    Plasmid

    #130935

    Purpose

    PiggyBac vector for DOX-inducible human HES7-IRES-EGFP expression in mammalian cells

    Depositor

    James Thomson

    Article

    Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

    Insert

    HES7 (HES7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJZC32

    Plasmid

    #62327

    Purpose

    sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. BCL9-BirA*

    Plasmid

    #109147

    Purpose

    To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

    Depositor

    Mariann Bienz

    Article

    van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

    Insert

    BCL9 (BCL9 Human)
    

    Use

    Tags

    BirA(R118G)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO2

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. LN-entry+NLS

    Plasmid

    #128011

    Purpose

    Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

    Depositor

    Julius Brennecke

    Article

    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

    Insert

    LN-3xFlag-SV40NLS
    

    Use

    Lamdan entry vector with nls to generate tetherin…

    Tags

    LN-3xFlag-SV40NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. B4GALT1-Dox

    Plasmid

    #124633

    Purpose

    Doxycycline-inducible B4GALT1 expression in mammalian cells

    Depositor

    Ron Weiss

    Article

    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

    Inserts

    Beta-(1,4)-Galactosyltransferase 1
    Tetracycline repressor A3 mutant
    TagFP635
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight and hEF1a

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-uTEV1-MAVS-mTA4

    Plasmid

    #171002

    Purpose

    HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[Y536delinsFIVLI]

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMT-MCS-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80058

    Purpose

    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSW002-PpsbA-mNeonGreen

    Plasmid

    #205017

    Purpose

    Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    PpsbA

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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