We narrowed to 7,377 results for: tet

1. pUC-hU6-crBFP_EF1a-BFP

   Plasmid

   #224785

   Purpose

   BFP targeting crRNA for RfxCas13d expressed from hU6 promoter and target BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Insert

   crBFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6 and hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC-hU6-CrEGFP_EF1a-BFP

   Plasmid

   #224786

   Purpose

   EGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Insert

   crBFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6 and hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Syn-ExSYTE.H64A

   Plasmid

   #216262

   Purpose

   inactive ExSYTE.H64A (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoter

   Depositor

   Susumu Tomita

   Article

   Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

   Insert

   ExSYTE P2A EGFP - Rat DTE
   

   Use

   AAV

   Mutation

   H64A mutation in each oTetR

   Available Since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AA440

   Plasmid

   #216036

   Purpose

   Fragmid fragment: (Cas protein) binds to TRE in absence of doxycycline

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   TetR_v1.1
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYG215

   Plasmid

   #200837

   Purpose

   Transcribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.

   Depositor

   Boris Görke

   Article

   Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.

   Insert

   glmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Bacterial

   Promoter

   P-LlacO-1

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. DsRed -7e

   Plasmid

   #191765

   Purpose

   Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   DsRed
   

   Tags

   N terminal fusion of negatively charged polypetod…

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette

   Plasmid

   #169743

   Purpose

   CMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   mScarlet-I
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   pCMV-tetO2

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pClgn1.1_Kctd19-3xHA

   Plasmid

   #173497

   Purpose

   Express Kctd19-3xHA under the mouse Clgn promoter.

   Depositor

   Masahito Ikawa

   Article

   Oura et al PLoS Genet. 2021 May 7;17(5):e1009412. d

   Insert

   Potassium Channel Tetramerization Domain Containing 19 (Kctd19 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pClgn1.1_3xFLAG-Kctd19

   Plasmid

   #173498

   Purpose

   Express 3xFLAG-Kctd19 under the mouse Clgn promoter.

   Depositor

   Masahito Ikawa

   Article

   Oura et al PLoS Genet. 2021 May 7;17(5):e1009412. d

   Insert

   Potassium Channel Tetramerization Domain Containing 19 (Kctd19 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-uTEV1-SQSfl

    Plasmid

    #171010

    Purpose

    HiLITR protease with full-length SQS targeting information (ER)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-SQS(FL)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    pTRE-tight (TetOn)

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. RA35

    Plasmid

    #141126

    Purpose

    Expresses dimeric LacI repressor (LacI-11) under Rhl promoter

    Depositor

    Matthew Bennett

    Article

    Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

    Inserts

    dimeric LacI (LacI-11) (lacI Synthetic)
    AiiA (AQ980_RS12585 )
    

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    truncated/dimeric version of LacI (missing last 1…

    Promoter

    engineered rhl promoter (responds to C4-HSL) and …

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-neo-His-MS2BP-G3BP1-S149A

    Plasmid

    #136009

    Purpose

    S149A G3BP1 inserted with MS2BP tagged on the N-terminus to use with the Tethering Assay (MS2)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Tags

    MS2BP

    Expression

    Mammalian

    Mutation

    S149A

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT3-RhoGC D380E

    Plasmid

    #100035

    Purpose

    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 533 nm.

    Depositor

    Daniel Oprian

    Article

    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

    Insert

    RhoGC D380E
    

    Tags

    1D4 (TETSQVAPA)

    Expression

    Mammalian

    Mutation

    D380E

    Promoter

    Adenovirus major late promoter

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT3-RhoGC D380N

    Plasmid

    #100034

    Purpose

    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 506 nm.

    Depositor

    Daniel Oprian

    Article

    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

    Insert

    RhoGC D380N
    

    Tags

    1D4 (TETSQVAPA)

    Expression

    Mammalian

    Mutation

    D380N

    Promoter

    Adenovirus major late promoter

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZS1[Iq:LacI-T]

    Plasmid

    #60756

    Purpose

    Contains PIq driving expression LacI-T, the Lactose inducible chimera with the TAN DBD.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Insert

    LacI-T
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    LacI without the tetramerization domain and the T…

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFAB1907

    Plasmid

    #47857

    Purpose

    BIOFAB reporter plasmid for measuring BBa_B1006 U10 termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    BBa_B1006 U10
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Added two extra U at the end of Bba_1006 poly-U t…

    Promoter

    pLTetO12

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFAB801

    Plasmid

    #47846

    Purpose

    BIOFAB reporter plasmid for measuring trp_att_L126 termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    trp_att_L126 terminator
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Loop mutation in trp Terminator

    Promoter

    pLTetO1

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-sgE1TSS-short-3'box

    Plasmid

    #92165

    Purpose

    sgRNA expression vector for Evx1as RNA tethering assay

    Depositor

    Xiaohua Shen

    Article

    Luo et al Cell Stem Cell. 2016 May 5;18(5):637-52.

    Insert

    Evx1as short isoform
    

    Use

    CRISPR

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

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19. hCathepsin L

    Plasmid

    #11250

    Purpose

    Mammalian expression of human cathepsin L

    Depositor

    Hyeryun Choe

    Article

    Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

    Has Service

    Cloning Grade DNA

    Insert

    cathepsin L (CTSL Human)
    

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. hCathepsin S

    Plasmid

    #11251

    Purpose

    Mammalian expression of human Cathepsin S

    Depositor

    Hyeryun Choe

    Article

    Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

    Insert

    cathepsin S (CTSS Human)
    

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits