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pPW3763 : CMVd3-HsIRE1dLKR-HaloTag

Plasmid

#185685

Purpose

Low-level transient expression of HsIRE1 lacking the linker, kinase, and RNase domains, with a C-terminal HaloTag.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 (ERN1 Human, Synthetic)

Tags

HaloTag

Expression

Mammalian

Mutation

Deletion of linker, kinase, and Rnase domains

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT-RP8.1

Plasmid

#189846

Purpose

Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 1.

Depositor

Stephen Ekker

Article

Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

Type

Empty backbone

Use

Cre/Lox

Tags

mRFP and tagBFP

Promoter

beta Actin

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-cmlc2-TagBFP

Plasmid

#117774

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-gcry-TagBFP

Plasmid

#117783

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-gcry-TagBFP

Plasmid

#117789

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-SynDIG1/Tmem90b [L42/17R]

Plasmid

#128632

Purpose

Mammalian Expression Plasmid of anti-SynDIG1/Tmem90b (Mouse). Derived from hybridoma L42/17.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SynDIG1/Tmem90b (Mouse) recombinant mouse monoclonal antibody (Syndig1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-TrpV3 [N15/4R]

Plasmid

#177501

Purpose

Mammalian Expression Plasmid of anti-TrpV3 (Rat). Derived from hybridoma N15/4.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TrpV3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv3 Rat)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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C2-58 pComb3xss anti-cFos nanobody

Plasmid

#145831

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C2-58 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-3 [N278/19R]

Plasmid

#114476

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-3 (Mouse). Derived from hybridoma N278/19.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-3 (Mus musculus) recombinant mouse monoclonal antibody (Syt3 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-TRIP8b (constant) [N212/17R]

Plasmid

#114557

Purpose

Mammalian Expression Plasmid of anti-TRIP8b (constant) (Rat). Derived from hybridoma N212/17.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TRIP8b (constant) (Rattus norvegicus) recombinant mouse monoclonal antibody (Pex5l Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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