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pCMV-myc-mIFITM3

Plasmid

#58458

Purpose

Expresses murine IFITM3 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Publication

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only
pTK157_mCherry-4.1G-CTD

Plasmid

#46359

Depositor

Iain Cheeseman

Publication

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G C-terminal domain (EPB41L2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

C-terminal domain from 886-1005 amino acids

Promoter

CMV

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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TALEN-ID12-C

Plasmid

#41477

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Tags

3xFLAG and WT FokI nuclease domain

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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TALEN-ID12-T

Plasmid

#41479

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Tags

3xFLAG and WT FokI nuclease domain

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV-831-1792

Plasmid

#40961

Depositor

Elizabeth Luna

Publication

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-830

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-1010-1792

Plasmid

#40959

Depositor

Elizabeth Luna

Publication

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-1009

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
TAL2324

Plasmid

#36750

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MAP2K4-TALEN_Left (Map2k4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2325

Plasmid

#36751

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MAP2K4-TALEN_Right (Map2k4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2342

Plasmid

#36762

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PTCH1-TALEN_Left (PTCH1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (T56D)

Plasmid

#24532

Depositor

Roger Davis

Publication

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(T56D) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed threonine 56 to aspartic acid

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Bmf (S74A)

Plasmid

#24258

Depositor

Roger Davis

Publication

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (S87A) (BMF Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 74 to Alanine

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (S58A)

Plasmid

#24249

Depositor

Roger Davis

Publication

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(S58A) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed serine 58 to alanine

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
M19 pCS-twhh-dsl-1myc

Plasmid

#17156

Depositor

Randall Moon

Publication

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

dorsalin-1 (GDF2 Chicken)

Tags

myc-tag and twhh promoter (D rerio)

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pFbx15(-544/-527)-TK-Luc

Plasmid

#13414

Depositor

Shinya Yamanaka

Publication

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Mutation

None

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf159

Plasmid

#12847

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Publication

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf159

Expression

Mammalian

Available since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
mEmerald-myo1e

Plasmid

#249264

Purpose

Fluorescently tagged myosin 1e

Depositor

Mira Krendel

Publication

Barger et al Nat Commun. 2019 Mar 19;10(1):1249. doi:

Insert

Myo1e (MYO1E Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pME-zFUCCI-P2A-H2A-tdiRFP (JDW 1380)

Plasmid

#242559

Purpose

Gateway middle entry clone containing a zebrafish FUCCI reporter followed by H2A tdiRFP reporter; For visualizing cell cycle state.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)

Use

Gateway Cloning

Available since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_Ckm_F9

Plasmid

#226140

Purpose

AAV construct for HITI insert with Ckm gRNA and hF9 transgene

Depositor

Christopher Nelson

Publication

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Inserts

F9 (F9 Human)
Ckm gRNA (Ckm Mouse)
EGFP

Use

AAV and CRISPR

Expression

Mammalian

Available since

Aug. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pMRX-IP-EGFP-LOV2-Cyb5a

Plasmid

#233348

Purpose

Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

Depositor

Noboru Mizushima

Publication

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

LOV2 domain of phototropin1
Cyb5a (Cyb5a Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

N-terminus is deleted (Cyb5a 95-134) and N538E

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-LAMP1-mScarleti

Plasmid

#228526

Purpose

Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-mScarleti in mammalian cells.

Depositor

Ginny Farias

Publication

Li et al Nat Commun. 2024 Dec 30;15(1):10829. doi

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP), Streptavidin-…

Expression

Mammalian

Promoter

Ubc

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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