We narrowed to 6,899 results for: tet
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Plasmid#217705PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGR7 (ADGRG7 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-415Available SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NIP45 wt
Plasmid#210266PurposeInducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.DepositorInsertNIP45 wt siRES (NFATC2IP Synthetic)
TagsmCherryExpressionMammalianMutationsiRESPromoterCMV/TetO2Available SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-NIP45 wt
Plasmid#210262PurposeInducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.DepositorInsertNIP45 wt siRES (NFATC2IP Synthetic)
TagsEGFPExpressionMammalianMutationsiRESPromoterCMV/TetO2Available SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENeCFPCNAL2 pc922
Plasmid#167563PurposeFor construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA).DepositorAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(363-393)
Plasmid#200314Purposebacterial expression of P53 CTDDepositorAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRA1SEGFPTcIfm
Plasmid#193777PurposeCassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selectionDepositorInsertEGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
UseSynthetic BiologyExpressionBacterialPromoterPR promoter and PLTetO-1Available SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTA1cI-T3
Plasmid#193789PurposeExpresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selectionDepositorInsertTruncated version of Lambda CI, named T3, where the first two codons are deleted
UseSynthetic BiologyExpressionBacterialPromoterPLTetO-1Available SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
SFRS17A
Plasmid#156010PurposeFor use in RBP tethering screenDepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
gSCPL35-mRFP
Plasmid#181960PurposeSCPL35 protein tagged with mRFP, under control of native promoterDepositorInsertSCPL35
TagsmRFPExpressionPlantPromoternative SCPL35Available SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1)
Plasmid#170488PurposeSPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1DepositorInsertFRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry
UseRetroviralExpressionMammalianAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB237.1
Plasmid#170031PurposeEncodes LacI-controlled expression of surface-displayed CCL19 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CCL19 Δ alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB235.2
Plasmid#170025PurposeEncodes LacI-controlled expression of surface-displayed CCL20 alpha-helix fragment and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CCL20 alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB237.2
Plasmid#170032PurposeEncodes LacI-controlled expression of surface-displayed CCL19 alpha-helix fragment and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CCL19 alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCh1753_S2F-IMCg_doxy-CMV_mChe-Cdc42E7-E63'UTR
Plasmid#118615Purposeexpression of mChe-tagged cdc42E7-E6-3'UTR (swap) under doxy-inducible CMV promoterDepositorInsertcdc42E7-E6 3'UTR (Cdc42 Mouse)
UseLentiviralTagsmCherryExpressionMammalianPromotertetON CMVAvailable SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-X1
Plasmid#115566PurposeGFP tethered to a single repeat of the Gcn5 consensus motifDepositorInsertGreen Fluorescent Protein
ExpressionBacterial and YeastMutationSingle Gcn5 consensus motif repeat tethered to C-…PromoterADH1Available SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTHFR (MTHFRA-c042)
Plasmid#110271PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoGC D380E
Plasmid#100035PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 533 nm.DepositorInsertRhoGC D380E
Tags1D4 (TETSQVAPA)ExpressionMammalianMutationD380EPromoterAdenovirus major late promoterAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoGC D380N
Plasmid#100034PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 506 nm.DepositorInsertRhoGC D380N
Tags1D4 (TETSQVAPA)ExpressionMammalianMutationD380NPromoterAdenovirus major late promoterAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHL 762
Plasmid#52990PurposepLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfpDepositorInsertsMicC sRNA
sodB
ompC
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutation−56 to +141 bp and −81 to +36 bpPromoterpLlacO-1 and pLtetO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1394
Plasmid#53009PurposepLtetO-1:RhyB, pCon:T7(st3) (133bps)::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
T7(st3) (133bps)
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −56 to +141 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only