We narrowed to 9,186 results for: mel

1. pDECKO_GFP_AAVS1

   Plasmid

   #141211

   Purpose

   pDECKO_GFP_AAVS1_1801. Targeting, non-essential locus control

   Depositor

   Rory Johnson

   Article

   Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

   Insert

   gRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-BRD9_N216A-PGK-Puro-IRES-GFP

   Plasmid

   #75116

   Purpose

   Retroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutation

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Mutation

   single amino acid mutation N216A

   Available Since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pDECKO_mCherry_AAVS1_1801

   Plasmid

   #159090

   Purpose

   targeting, non-essential locus control

   Depositor

   Rory Johnson

   Article

   Esposito et al Cell Genom. 2022 Aug 22;2(9):100171. doi

   Insert

   gRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MSCV-BRD9_BET-PGK-Puro-IRES-GFP

   Plasmid

   #75120

   Purpose

   Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Mutation

   Bromodomain replaced with the first bromodomain o…

   Available Since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

5. INCTbiosyn-p35S-GFP(rc)phiC31

   Plasmid

   #127527

   Purpose

   Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
   

   Expression

   Plant

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. INCTbiosyn-p35S-GFP(rc)Bxb1

   Plasmid

   #127528

   Purpose

   Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
   

   Expression

   Plant

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459-dCTNNA1

   Plasmid

   #176032

   Purpose

   A knock-out vector for dog CTNNA1 (alpha-1-catenin)

   Depositor

   Michiyuki Matsuda

   Article

   Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

   Insert

   A gRNA targeting the dog CTNNA1 (alpha-1-catenin) gene and the cDNA of Cas9 (CTNNA1 )
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUW-CAGGS-ERAS

   Plasmid

   #52416

   Purpose

   Lentiviral vector encoding constitutive expression of human ERAS cDNA

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   ERAS (ERAS Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. BCL2-pcw107-V5

   Plasmid

   #64630

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   BCL2 (transcript variant alpha) (BCL2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA-DDX11-Flag-KAE

    Plasmid

    #120728

    Purpose

    Expression of a Flag-tagged version of DDX11 KAE mutant in mammalian cells

    Depositor

    Francesca Pisani

    Article

    Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

    Insert

    DDX11 KAE mutant (DDX11 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Substitution of E201 and Y202 with K and A

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1α1.1-Chronos-GFP

    Plasmid

    #122098

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1α (1.1 kb short version)

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-DDX11-Flag-KAK

    Plasmid

    #120729

    Purpose

    Expression of a Flag-tagged version of DDX11 KAK mutant in mammalian cells

    Depositor

    Francesca Pisani

    Article

    Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

    Insert

    DDX11 KAK mutant (DDX11 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Substitution of E201, Y202 and E203 with K, A and…

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR223-ZNF698

    Plasmid

    #88666

    Purpose

    Donor Vector containing ZNF698 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF698 (ZFP57 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEAHISPApqsE

    Plasmid

    #97019

    Purpose

    Expressing Pseudomonas aeruginosa pqsE protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Quinolone signal response protein
    

    Tags

    N-ter TEV protease cleavable 6HIs

    Mutation

    none

    Promoter

    T7

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221-HES5

    Plasmid

    #101409

    Purpose

    Donor Vector containing HES5 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    HES5 (HES5 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEAHISMRSA2676

    Plasmid

    #96970

    Purpose

    expression of methicillin-resistant Staphylococcus aureus orf 2676

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Sar2676
    

    Tags

    N-ter TEV protease cleavable 6His

    Mutation

    none

    Promoter

    T7

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEAHISMRSAMVAK

    Plasmid

    #97000

    Purpose

    expression of methicillin-resistant Staphylococcus aureus MVAK protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Mevalonate kinase
    

    Tags

    N-ter TEV protease cleavable 6His

    Mutation

    none

    Promoter

    T7

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TRCN0000297275

    Plasmid

    #78152

    Purpose

    shCDK4 targeting 3UTR

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    CDK4 (CDK4 Human)
    

    Use

    RNAi

    Promoter

    hU6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

19. MSCV-BRD9_BRG1BD-PGK-Puro-IRES-GFP

    Plasmid

    #75121

    Purpose

    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRG1 bromodomain

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Mutation

    Bromodomain replaced with the bromodomain of BRG1

    Available Since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

20. MSCV-BRD9_BRD1BD-PGK-Puro-IRES-GFP

    Plasmid

    #75119

    Purpose

    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD1 bromodomain

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Mutation

    Bromodomain replaced with the bromodomain of BRD1

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only