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pDL0_198

Plasmid

#210865

Purpose

C1_Pyrearinus termitilluminans beetle luciferase ('Eluc' version - human-codon optimized)

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

Pyrearinus termitilluminans beetle luciferase

Use

Luciferase and Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_162

Plasmid

#210775

Purpose

C1_Equine rhinitis A virus_E2A skipping peptide (E2A)

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

Equine rhinitis A virus_E2A skipping peptide

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_033

Plasmid

#210755

Purpose

PU_Zea mays EF1a promoter

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

Zea Mays Elongation factor 1A promoter

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_028

Plasmid

#210759

Purpose

PU_6 x Lac operator sequence 35S minimal fusion promoter (pOp6)

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

6 x Lac Operator sequence

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_029

Plasmid

#210761

Purpose

SC_Oryza sativa codon-optimized LHGR-N(Ligand binding domain and rat glucocorticoid receptor (rcoLHGR-N)

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

rice codn optimized LHGRN

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_044

Plasmid

#210764

Purpose

T_Arabidopsis thaliana heat shock protein 18.2 terminator (HspT)

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

Terminator of Arabidopsis thaliana heat shock protein 18.2

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pXYB033

Plasmid

#239387

Purpose

Super folding Green Fluorescence Protein (sfGFP)

Depositor

Xiaohan Yang

Article

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Insert

Superfolder GFP (gfp )

Use

Synthetic Biology

Expression

Plant

Promoter

35S

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLUEF1 IRES GFP (backbone)

Plasmid

#224466

Purpose

Ef1a downstream of a ubiquitous chromatin opening element drives expression of GOI and IRES GFP

Depositor

Robert Kerbel

Article

Khan et al Cancer Res. 2025 Apr 14. doi: 10.1158/00

Type

Empty backbone

Use

Lentiviral

Promoter

Ef1a

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pGY18

Plasmid

#221708

Purpose

Empty Cas9 vector for multiplex gene editing in fungi

Depositor

Kyle Pomraning

Article

Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

Type

Empty backbone

Use

CRISPR; Aspergillus

Expression

Bacterial

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET-His-E1GFP

Plasmid

#233885

Purpose

Bacterial Expression of recombinant His-tagged E1GFP

Depositor

Francesco Cardarelli

Article

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

E1GFP

Tags

His

Expression

Bacterial

Mutation

T65S and T203Y compared to EGFP

Promoter

T7 promoter

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO_24xGCN4_PCC_24xPP7

Plasmid

#236094

Purpose

Constitutively encodes 24xGCN4-PCC-24xPP7 stem loops, for visualization of the Pcc mRNA.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

24xGCN4-PCC-24xPP7

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO_24xGCN4_MUT_24xPP7

Plasmid

#236093

Purpose

Constitutively encodes 24xGCN4-MUT-24xPP7 stem loops, for visualization of the Mut mRNA.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

24xGCN4-MUT-24xPP7

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_GFP

Plasmid

#235611

Purpose

Encodes GFP. For use in transfections as a negative control without epitope tags.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

GFP

Expression

Mammalian

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_GFP

Plasmid

#235632

Purpose

Encodes V5-GFP with an N-terminal nuclear export sequence. Used as a no-ALIBi negative control.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

GFP

Tags

NES-V5

Expression

Mammalian

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF9-Pct3-(–)-Eldh2(cHis6)-Accs(ElA2)

Plasmid

#233868

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh2

Tags

His

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pISEre1reRNA

Plasmid

#226821

Purpose

Expressing ISEre1 guide RNA targeting maeB site controled by PJ23119

Depositor

Sheng Yang

Article

Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

Insert

ISEre1 none target guide RNA

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN16-HcdhA(cHis8)BC-partial cluster(ElA2)

Plasmid

#225328

Purpose

Expresses Eggerthella lenta A2 Hcdh partial gene cluster (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Hcdh partial gene cluster

Tags

His8

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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GB4003

Plasmid

#215248

Purpose

Module for the constitutive expression of PIF6 and PhyB:VP16

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

PIF6-PhyB:VP16

Use

Synthetic Biology

Mutation

V234L in PIF6- please see dep. sequence, BsaI and…

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN6-Elen0497-ElVadh-noAccs(ElDSM2243)

Plasmid

#225314

Purpose

Expresses Eggerthella lenta DSM 2243 El Vadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

El Vadh (Elen_0497 Eggerthella lenta)

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN20-Pct5-GPA_25810-(+)-Eldh1(GpDSM19378)

Plasmid

#225306

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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