We narrowed to 2,242 results for: D10

1. pFAB3914

   Plasmid

   #47817

   Purpose

   BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD10 efficiency.

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   promoter 14 and BCD10
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   see insert

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEntry_HCoV-OC43-Hemagglutinin-esterase

   Plasmid

   #168943

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-OC43-Hemagglutinin-esterase (HE )
   

   Use

   Gateway-compatible entry vector

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDG335

   Plasmid

   #100899

   Purpose

   SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9 Nickase
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A mutant converts to Nickase

   Promoter

   CBh and U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ265E6

   Plasmid

   #213469

   Purpose

   Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

   Depositor

   Yiping Qi

   Article

   Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

   Insert

   TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
   

   Use

   CRISPR; Gateway compatible tada-cda-32aa linker-z…

   Tags

   No

   Expression

   Plant

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEntry_HCoV-HKU1-Hemagglutinin-esterase

   Plasmid

   #168902

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-HKU1-Hemagglutinin-esterase (HE )
   

   Use

   Gateway-compatible entry vector

   Mutation

   Codon optimized (H.sapiens)

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-eA3A-BE5-(NG)-PX458-EGFP

   Plasmid

   #229687

   Purpose

   CRISPR CBE plasmid (C to T edits): eA3A-BE5-NG engineered to include the sgRNA cloning backbone from PX458 and EGFP. Includes BbsI sites for sgRNA cloning downstream of U6 promoter.

   Depositor

   Deborah Marsh

   Article

   Dickson et al Hum Mol Genet. 2023 Jun 27:ddad105. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ265E7

   Plasmid

   #213470

   Purpose

   Gateway entry clone (attL1 & attR5) TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

   Depositor

   Yiping Qi

   Article

   Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

   Insert

   TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI
   

   Use

   CRISPR; Gateway compatible tada-cdd-32aa linker-z…

   Tags

   No

   Expression

   Plant

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ265E8

   Plasmid

   #213471

   Purpose

   Gateway entry clone (attL1 & attR5) TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

   Depositor

   Yiping Qi

   Article

   Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

   Insert

   TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI
   

   Use

   CRISPR; Gateway compatible tada-cdd_v106w-32aa li…

   Tags

   No

   Expression

   Plant

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEntry_HCoV-NL63-Protein3

   Plasmid

   #168923

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-NL63-Protein3 (HCNV63gp3 )
   

   Use

   Gateway-compatible entry vector

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEntry_SARS-CoV-2-Spike

    Plasmid

    #168891

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    SARS-CoV-2-Spike (S )
    

    Use

    Gateway-compatible entry vector

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJOG252

    Plasmid

    #80584

    Purpose

    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJOG253

    Plasmid

    #80585

    Purpose

    recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:PAT-tnos
    p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
    pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHSN501

    Plasmid

    #50589

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    zCas9D10A
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG and NLS

    Mutation

    Cas9D10A nickase, was derived from the zCas9 and …

    Promoter

    2×35Sp and AtU6-26p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. BE3-P2A-EGFP (pJUL977)

    Plasmid

    #123612

    Purpose

    CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3-P2A-EGFP
    

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CAG

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBUN501

    Plasmid

    #50582

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    zCas9D10A
    gRNA scaffold
    

    Use

    CRISPR; Plant expression

    Tags

    3xFLAG and NLS

    Mutation

    Cas9D10A nickase, was derived from the zCas9 and …

    Promoter

    AtU6-26p and Ubi1p

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

    Plasmid

    #123616

    Purpose

    CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    NLS-nCas9-NLS-P2A-EGFP
    

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

    Plasmid

    #123611

    Purpose

    CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    nCas9-UGI-NLS-P2A-EGFP
    

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CAG

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

    Plasmid

    #123615

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(R33A/K34A)-P2A-EGFP
    

    Expression

    Mammalian

    Mutation

    R33A/K34A in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

    Plasmid

    #123614

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(R33A)-P2A-EGFP
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. BE3(E63Q)-P2A-EGFP (pRZ189)

    Plasmid

    #123613

    Purpose

    CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    BE3(E63Q)-P2A-EGFP
    

    Expression

    Mammalian

    Mutation

    E63Q in rAPOBEC1, D10A in SpCas9

    Promoter

    CAG

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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