We narrowed to 4,419 results for: crispr c plasmids
-
Plasmid#77861Purpose3rd generation lentiviral gRNA plasmid targeting human PRKDCDepositorAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by
-
pCMV-T7-SpRY-P2A-mScarlet (MNW002)
Plasmid#174137PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarletDepositorInserthuman codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationSpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…PromoterCMV and T7Available sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJEP302-pAAV-CMV-dSaCas9-KRAB-pA
Plasmid#113677PurposeA CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.DepositorInsertde-catalyzed SaCas9
UseAAV and CRISPRTagsKRAB and NLSAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
ABE7.10-F148A
Plasmid#132946PurposeGene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.DepositorInsertABE7.10(F148A)
UseCRISPR; Base editorExpressionMammalianMutationABE7.10(F148A)Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAP635-2
Plasmid#99485PurposeMyc::TEV::3XFlag::Dendra2::3XFlag::TEV::MycDepositorInsertMyc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc
ExpressionBacterialAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-eA3A-BE5-(NG)-PX458-EGFP
Plasmid#229687PurposeCRISPR CBE plasmid (C to T edits): eA3A-BE5-NG engineered to include the sgRNA cloning backbone from PX458 and EGFP. Includes BbsI sites for sgRNA cloning downstream of U6 promoter.DepositorTypeEmpty backboneUseCRISPRTagsEGFPExpressionMammalianAvailable sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDD316
Plasmid#137020PurposemTurq2-GLO^SEC^2xHA vector with ccdB markers for cloning homology armsDepositorInsertmTurq2-GLO^SEC^2xHA + ccdB
UseCRISPR and Cre/LoxTags2xHA and C. elegans GLO-optimized mTurq2ExpressionWormAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZNF416-donor
Plasmid#112372PurposeCRISPR donor plasmid to tag human transcription factor ZNF416 with GFPDepositorInsertZNF416 homology arms flanking EGFP-IRES-Neo cassette (ZNF416 Human)
UseCRISPRMutationhg38:19:57572882:A>G,hg38:19:57572932:C>G,h…Available sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLX-donor
Plasmid#112370PurposeCRISPR donor plasmid to tag human transcription factor MLX with GFPDepositorInsertMLX homology arms flanking EGFP-IRES-Neo cassette (MLX Human)
UseCRISPRMutationhg38:17:42570798:G>A, hg38:17:42570950:T>G,…Available sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUSF2-donor
Plasmid#112361PurposeCRISPR donor plasmid to tag human transcription factor USF2 with GFPDepositorInsertUSF2 homology arms flanking EGFP-IRES-Neo cassette (USF2 Human)
UseCRISPRMutationhg38:19:35278400:T>A, hg38:19:35278455:G>CAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXD1-donor
Plasmid#112350PurposeCRISPR donor plasmid to tag human transcription factor MXD1 with GFPDepositorInsertMXD1 homology arms flanking EGFP-IRES-Neo cassette (MXD1 Human)
UseCRISPRMutationhg38:2:69937537:A>C,rs3771530Available sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDLX1-donor
Plasmid#112343PurposeCRISPR donor plasmid to tag human transcription factor DLX1 with GFPDepositorInsertDLX1 homology arms flanking EGFP-IRES-Neo cassette (DLX1 Human)
UseCRISPRMutationhg38:2:172087403:G>C,hg38:2:172087433:G>A,h…Available sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)
Plasmid#139993PurposeCMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationxCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AXL gRNA (BRDN0001146797)
Plasmid#76700Purpose3rd generation lentiviral gRNA plasmid targeting human AXLDepositorAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RHO-IntC-dCas9C-VPR-polyA
Plasmid#166692PurposeExpress C-terminal part of split dCas9-VPRDepositorInsertIntC-dCas9C-VPR
UseAAV and CRISPRExpressionMammalianPromotershort human rhodopsin promoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dCas9v2
Plasmid#103140PurposeTetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready siteDepositorInsertdCas9-VPR
ExpressionYeastMutationRemoved scaffold gRNA from original plasmidAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)
Plasmid#133789PurposeCAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)DepositorInserthuman codon optimized CjeCas9
TagsNLS(SV40)-3xFLAGExpressionMammalianMutationn/aPromoterCAGAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAT27
Plasmid#204308PurposeFor generating targeting inversions. C-terminal split GFP, expressed in flight muscles.DepositorPublicationInsertMHC-GFPc
UseCRISPRPromoterMHCAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBHM2646
Plasmid#229530PurposeCarries mAID_opt tag, empty sgRNA, and NATR markerDepositorInsertmAID_opt - empty sgRNA - NATR
Tags2xFLAGExpressionBacterial and YeastAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBHM2650
Plasmid#229992PurposeCarries AID* tag, empty sgRNA, and HYGR markerDepositorInsertAID* - empty sgRNA - HYGR
Tags2xFLAGExpressionBacterial and YeastAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only