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Lenti-117G-hyeA3A-BE4max

Plasmid

#157946

Purpose

Lentiviral expression of hyeA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

Lenti -117G-hyeA3A-BE4max

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N57G

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRhaB-8xHis-ABE8e-eVRQR (LLH551)

Plasmid

#242658

Purpose

pRhaB promoter expression plasmid for ABE8e-eVRQR with an N-terminal His8-tag

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pRhaB-8xHis-BPNLS-TadA8e-SpCas9(D10A)-eVRQR-BPNLS

Use

CRISPR

Tags

8x-His

Expression

Bacterial

Mutation

eVRQR mutations in SpCas9(S55R/D1135V/G1218R/R133…

Promoter

RhaB

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-pLenti-ACTA2-R179H (LLH647)

Plasmid

#242683

Purpose

Plasmid for expression of ACTA2-R179H cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-R179H_cDNA

Use

Lentiviral

Expression

Mammalian

Mutation

ACTA2-R179H

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-pLenti-ACTA2-L180M (LLH673)

Plasmid

#242686

Purpose

Plasmid for expression of ACTA2-L180M cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-L180M_cDNA

Use

Lentiviral

Expression

Mammalian

Mutation

ACTA2-L180M

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-pLenti-ACTA2-R179 (WT) (LLH661)

Plasmid

#242682

Purpose

Plasmid for expression of ACTA2-R179 (wild-type) cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-R179_cDNA

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-pLenti-ACTA2-L180V (LLH669)

Plasmid

#242685

Purpose

Plasmid for expression of ACTA2-L180V cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-L180V_cDNA

Use

Lentiviral

Expression

Mammalian

Mutation

ACTA2-L180V

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-pLenti-ACTA2-D181G (LLH681)

Plasmid

#242687

Purpose

Plasmid for expression of ACTA2-D181G cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-D181G_cDNA

Use

Lentiviral

Expression

Mammalian

Mutation

ACTA2-D181G

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-pLenti-ACTA2-L180L (LLH677)

Plasmid

#242688

Purpose

Plasmid for expression of ACTA2-L180L cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-L180L_cDNA

Use

Lentiviral

Expression

Mammalian

Mutation

ACTA2-L180L

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-pLenti-ACTA2-M178V (LLH709)

Plasmid

#242684

Purpose

Plasmid for expression of ACTA2-M178V cDNA from a lentiviral cassette

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pLenti-ACTA2-M178V_cDNA

Use

Lentiviral

Expression

Mammalian

Mutation

ACTA2-M178V

Promoter

CMV

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_V290A

Plasmid

#213522

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation V290A

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed V290 to A

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_N306K

Plasmid

#213523

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation N306K

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV2-NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed N306 to K

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

Plasmid

#213524

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to S

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

Plasmid

#213525

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to H

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

Plasmid

#213526

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed H424 to R

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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RALA V25L

Plasmid

#122927

Purpose

Bacterial expression of RALA V25L

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

V25L

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA V25M

Plasmid

#122928

Purpose

Bacterial expression of RALA V25M

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

V25M

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA D130G

Plasmid

#122929

Purpose

Bacterial expression of RALA D130G

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

D130G

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA S157A

Plasmid

#122930

Purpose

Bacterial expression of RALA S157A

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

S157A

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA R176X

Plasmid

#122931

Purpose

Bacterial expression of RALA R176X

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Mutation

R176X

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RALA WT

Plasmid

#122925

Purpose

Bacterial expression of RALA WT

Depositor

Greg Cooper

Article

Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

Insert

RALA (RALA Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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