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We narrowed to 11,357 results for: Pol

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Showing: 6801 - 6820 of 11357 results

  1. MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

    Plasmid
    #131730
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    TagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    TagBFP2-V5 tag
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    131730-plasmid-map-sequence-id-318177

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  2. pFastBac1 human full-length CENP-E-mNeon-6xHis

    Plasmid
    #180484
    Purpose
    pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells
    Depositor
    Julie Welburn
    Article
    Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1
    Insert
    CENP-E (CENPE Human, human, Synthetic)
    Tags
    mNeon-6xHis
    Expression
    Insect
    Promoter
    polyhedrin promoter
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    180484-plasmid-map-sequence-id-377471

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  4. pBactin-PACT-mOrange2-hCM1

    Plasmid
    #196868
    Purpose
    Expression of Pericentrin-AKAP450 centrosomal targeting (PACT) domain fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). PACT targets CM1 to the centrosome
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    PACT-mOrange2-hCM1 (Akap9 Rat)
    Expression
    Mammalian
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196868-plasmid-map-sequence-id-391838

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  6. pBactin-Nedd1-mOrange2-hCM1

    Plasmid
    #196863
    Purpose
    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-hCM1 (Nedd1 Rat, Human)
    Expression
    Mammalian
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196863-plasmid-map-sequence-id-391832

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  7. pBetaActin-FKBP-mScarlet-Dync1h1motor

    Plasmid
    #191330
    Purpose
    Expresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
    Expression
    Mammalian
    Mutation
    DYNC1H1 motor domain aa 1453-4644
    Available Since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    191330-plasmid-map-sequence-id-381732

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  10. pEcRNAP5

    Plasmid
    #128939
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z
    Depositor
    Seth Darst
    Article
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoAppx234-241-(His)10 (rpoA Escherichia coli)
    RpoB
    RpoC
    RpoZ
    Tags
    His
    Expression
    Bacterial
    Mutation
    Residues 234-241 are substituted for a PreScissio…
    Promoter
    T7
    Available Since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    128939-plasmid-map-sequence-id-254599

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  12. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

    Plasmid
    #119864
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Tags
    HA-tagged biotin ligase (BirA), Thosea asigna vir…
    Expression
    Bacterial
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    119864-plasmid-map-sequence-id-239418

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  18. LEGO-AP1x3-GM55

    Plasmid
    #217414
    Purpose
    Luciferase expression vector with 3 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x3
    Depositor
    Peter Cockerill
    Insert
    Luciferase, GFP
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    3 AP-1 sites, Human -55 to +28 minimal CSF2 promo…
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217414-plasmid-map-sequence-id-434967

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Showing: 6801 - 6820 of 11357 results