We narrowed to 12,189 results for: LEA
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Plasmid#135940PurposeExpresses AUF1p40 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
TagsFlag2 and His6ExpressionBacterialPromoterT7Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-3
Plasmid#126054PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1
Plasmid#129706PurposeExpress cre-dependent KASH-EGFP and Gmeb1 shRNADepositorInsertKASH-EGFP
UseAAVTagsEGFPExpressionMammalianPromoterU6Available sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-1
Plasmid#126056PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-AktAR2-D
Plasmid#125196PurposeSB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporterDepositorInsertAktAR2-D
UseTransposonTags6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…ExpressionMammalianMutationFoxO1 Thr-24 changed to Val rendering this inacti…PromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mTRAF3ip3-CFP
Plasmid#125181PurposeExpresses mouse TRAF3IP3 in mammalian cellsDepositorInsertTRAF3ip3 (Traf3ip3 Mouse)
TagsCFPExpressionMammalianMutationK87R, M283I, S359P, A506G- please see depositor c…PromoterCMVAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT5G16900 O2_pECIA14
Plasmid#114762PurposePrey vector AT5G16900 O2_pECIA14 should be used with bait vector AT5G16900 O2_pECIA2.DepositorInsertAT5G16900 (AT5G16900 Mustard Weed)
ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-PCDHGC5
Plasmid#119935PurposeProtocadherin gammaC5 expression (Myc-tag is at the linker between EC1 and EC2 extracellular cadherin repeats)DepositorInsertPCDHGC5 (Pcdhgc5 Rat)
Tags9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…ExpressionMammalianAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-delta/gamma2SIL
Plasmid#119731PurposeGABAA receptor expression (chimeric rat delta subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminusDepositorTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-delta
Plasmid#118037PurposeGABAA receptor expression (delta subunit) Myc-tag near N-terminusDepositorInsertGABRD (Gabrd Rat)
TagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEPOR1CB0113
Plasmid#117552PurposeLevel 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
WNK3 (WNK3B-c016)
Plasmid#110252PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-H698N
Plasmid#92242PurposeExpress H698N mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-H698N mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationHistidine 698 to AsparaginePromoterT7 promoterAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMGF170
Plasmid#96997Purposeish1-mCherry insertion cassette with NAT resistance geneDepositorInsertish1 (ish1 Fission Yeast)
TagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + NAT r…PromoterN/AAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mRuby2 LIC cloning vector (MBP-mRuby2)
Plasmid#86933PurposepET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mRuby2DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), MBP, TEV cleavage site, a…ExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
5E16 (PfPKG, PF14_0346)
Plasmid#79518PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPF14_0346
Tags6xHis and TEV cleavage siteExpressionBacterialPromoterT7-lacOAvailable sinceJune 15, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLL3.7 shRNA against CRTC1
Plasmid#72710PurposeLentiviral expression of shRNA against CRTC1 (3rd generation)DepositorInsertCRTC1
UseLentiviralTagsCMV-EGFPExpressionMammalianPromoterU6Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
5C0M
Plasmid#69962PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSGF29:JMC01N-E01:C207060 (SGF29 Human)
TagsHis and TEV cleavage siteExpressionBacterialPromoterT7-lacOAvailable sinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pONL-N1_3xNLS
Plasmid#65674PurposeExpresses NLS-ONL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsONLExpressionMammalianPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB695
Plasmid#60144PurposeDisintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertGrLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only