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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,067 results for: LEA

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Showing: 6821 - 6840 of 14067 results

  2. FLuc-N-HiBiT

    Plasmid
    #207117
    Purpose
    Bacterially expressed HiBiT-FLuc for Ni-NTA purification
    Depositor
    James Inglese
    Publication
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    luciferin 4-monooxygenase
    Tags
    HiBiT Tag and His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207117-plasmid-map-sequence-id-417172

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  3. IK349

    Plasmid
    #233655
    Purpose
    Dox-inducible red light-inducible Flp-C; Flp-397C-PhyA
    Depositor
    Wilson Wong
    Publication
    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Flp-397C-PhyA
    Expression
    Mammalian
    Mutation
    None
    Promoter
    TRE3G
    Available since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    233655-plasmid-map-sequence-id-464615

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  4. IK350

    Plasmid
    #233656
    Purpose
    Dox-inducible red light-inducible Flp-N; Flp-N396-FHY1
    Depositor
    Wilson Wong
    Publication
    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Flp-N396-FHY1
    Expression
    Mammalian
    Mutation
    None
    Available since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    233656-plasmid-map-sequence-id-464618

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  5. CT769

    Plasmid
    #233661
    Purpose
    Split Flp on same plasmid; PhyA-Flp-397-Flp-396-FHY1
    Depositor
    Wilson Wong
    Publication
    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PhyA-Flp-397-Flp-396-FHY1
    Expression
    Mammalian
    Mutation
    None
    Available since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    233661-plasmid-map-sequence-id-463755

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  13. pBactin-mOrange2-NEDD1-gTBD

    Plasmid
    #196858
    Purpose
    Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mOrange2-N-gTBD (NEDD1 Human)
    Expression
    Mammalian
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    196858-plasmid-map-sequence-id-391826

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  14. pBactin-PACT-mOrange2-hCM1

    Plasmid
    #196868
    Purpose
    Expression of Pericentrin-AKAP450 centrosomal targeting (PACT) domain fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). PACT targets CM1 to the centrosome
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PACT-mOrange2-hCM1 (Akap9 Rat)
    Expression
    Mammalian
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196868-plasmid-map-sequence-id-391838

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  15. pBactin-AcGFP-128-425-Akap9

    Plasmid
    #196871
    Purpose
    Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to AcGFP. Used to displace endogenous Akap9 from Golgi
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AcGFP-Akap9 (128-425) (Akap9 Rat)
    Expression
    Mammalian
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196871-plasmid-map-sequence-id-391844

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  16. pCAG-Halo-Tag-128-425-Akap9

    Plasmid
    #196873
    Purpose
    Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to Halo-Tag. Used to displace endogenous Akap9 from Golgi
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Halo-Tag-Akap9 (128-425) (Akap9 Rat)
    Expression
    Mammalian
    Promoter
    Quimeric CAG
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196873-plasmid-map-sequence-id-390445

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  17. pBactin-Nedd1-mOrange2-hCM1

    Plasmid
    #196863
    Purpose
    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC
    Depositor
    Frank Bradke
    Publication
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nedd1-mOrange2-hCM1 (Nedd1 Rat, Human)
    Expression
    Mammalian
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196863-plasmid-map-sequence-id-391832

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  19. AV15_pCAG.Cas9.gRNA.S1

    Plasmid
    #199259
    Purpose
    Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SpCas9 nuclease
    AAVS1-targeting gRNA
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG promoter and RNA polymerase III promoter for …
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199259-plasmid-map-sequence-id-391662

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Showing: 6821 - 6840 of 14067 results