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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,957 results for: pET

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Showing: 6841 - 6860 of 9957 results

  1. BAT8 (3RJW)

    Plasmid
    #28125
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    BAT8 (EHMT2 Human)
    Tags
    His-tag with integrated thrombin site
    Expression
    Bacterial
    Mutation
    contains amino acid residues 913-1193.
    Available since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. HLTF

    Plasmid
    #28152
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    helicase-like transcription factor (HLTF Human)
    Tags
    thrombin cleavage
    Expression
    Bacterial
    Mutation
    Contains HIRAN domain (residues 51 to 171).
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles

  4. pKK-RNAi-nucEGFPmiR-mCherry-TEV

    Plasmid
    #105808
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal mCherry.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    mCherry-TEV
    Expression
    Mammalian
    Available since
    May 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105808-plasmid-map-sequence-id-274623

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  5. pDC183

    Plasmid
    #106415
    Purpose
    Co-expresses trans SNARE complex with pQZ210 in E. coli
    Depositor
    Axel Brunger
    Publication
    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d
    Inserts
    SNAP25 fragment 141-204 (Snap25 Rat, Synthetic)
    SNAP25 Fragment 7-83 (Snap25 Rat, Synthetic)
    Expression
    Bacterial
    Mutation
    Fragment 7-83, Codon optimization and Fragment 14…
    Promoter
    T7
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106415-plasmid-map-sequence-id-204169

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  6. pKK-RNAi-nucEGFPmiR-TEV-mCherry

    Plasmid
    #105812
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-mCherry
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105812-plasmid-map-sequence-id-203423

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  7. PHF20 (3QII)

    Plasmid
    #32039
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PHF20 (PHF20 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Tudor domain 2 only (amino acids 83-150)
    Promoter
    T7
    Available since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. pT7-agrBD-IV

    Plasmid
    #53440
    Purpose
    Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV
    Depositor
    Cynthia Collins
    Publication
    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003
    Insert
    agrBD locus (with D29Y mutation in agrD)
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    D to Y mutation of residue 29 of the agrD gene (c…
    Promoter
    T7-lac
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    53440-plasmid-map-sequence-id-83235

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  11. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

    Plasmid
    #105805
    Purpose
    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-Cerulean; ORF2: TEV-Venus
    Expression
    Mammalian
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105805-plasmid-map-sequence-id-207906

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  13. pBJG1 T7-8xHis-OGT 5N5A

    Plasmid
    #154280
    Purpose
    Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Publication
    Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; bact…
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154280-plasmid-map-sequence-id-304840

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  14. CXXC1

    Plasmid
    #32045
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CXXC1 (CXXC1 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    CXXC domain (amino acids residues 161-222)
    Available since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pHL 1032

    Plasmid
    #52997
    Purpose
    pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Publication
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    ompC
    sodB
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon a…
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

    Plasmid
    #169168
    Purpose
    Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.
    Depositor
    Sascha Martens
    Publication
    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017
    Insert
    MAP1LC3B (MAP1LC3B Human)
    Tags
    6X Histidine Tag, TEV cleavage site, mCherry
    Expression
    Bacterial
    Promoter
    T7 lac promoter
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    169168-plasmid-map-sequence-id-332048

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  18. GRLF1

    Plasmid
    #36195
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GRLF1 (Codon Devices Synthesized) (ARHGAP35 Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains the Ras homolog domain only (amino acid …
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 6841 - 6860 of 9957 results