We narrowed to 13,416 results for: sequence
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Plasmid#23215DepositorInsertCox4 (COX4 Budding Yeast)
UseRetroviralTagsDsRedMutationCox4 mitochondrial targeting sequence fused to Ds…Available SinceJuly 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;mafbbCE1-basEgfp
Plasmid#90137Purposecontains mafbb enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertmafbbE1basEgfp
TagsEGFPExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmEGFP-C
Plasmid#113751PurposeGateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags3xmEGFPAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
AP568-3
Plasmid#70048Purposeco-expression of Cas9 and crRNA 589 target sequenceDepositorInsertsgRNA for dpy‐10
UseCRISPRExpressionWormAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
IRE-TPI-wildtype-TREAT
Plasmid#196923PurposeThe coding sequence of Triose phosphate isomerase gene was fused downstream Renilla in the ORFDepositorInsertRenilla luciferase
ExpressionBacterial and MammalianAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
IRE-TREAT
Plasmid#196921PurposeThe 5UTR of the ferritin heavy chain mRNA was cloned upstream of the Renilla coding sequence in the ΔIRE-TREAT reporterDepositorInsertRenilla luciferase
ExpressionBacterial and MammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A
Plasmid#140451PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A mutantDepositorInsertGRM3 (GRM3 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanineAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV0764
Plasmid#133520PurposeExpression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
AP568-2
Plasmid#70047Purposeco-expression of Cas9 and crRNA 589 target sequenceDepositorInsertsgRNA for dpy‐10
UseCRISPRExpressionWormAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmRuby-N
Plasmid#113754PurposeGateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags3xmRuby2Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-ancestral-retroCHMP3-HA
Plasmid#154248Purposeexpresses ancestral New World monkey retroCHMP3 in mammalian cellsDepositorInsertreconstructed ancestral sequence of New World monkey retroCHMP3
TagsHAExpressionMammalianPromoterEF1-alphaAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-FAK
Plasmid#74478PurposeThis construct was engineered for the purpose of cloning and sequencing Focal Adhesion Kinase.DepositorAvailable SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV0773
Plasmid#133519PurposeExpression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIK198
Plasmid#65629Purpose"flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.DepositorTypeEmpty backboneUseCRISPRExpressionWormPromoterU6Available SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Paxillin-KM-C-10
Plasmid#57654PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertPaxillin KM
TagstdEosExpressionMammalianMutationMutation to remove Kozak sequencePromoterCMVAvailable SinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCoofy62
Plasmid#122014PurposeMammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionMammalianAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;mafbbCE1-P1Egfp
Plasmid#90155Purposecontains mafbb enhancer sequence, and mafbb specific gene promoter driving expression of eGFPDepositorInsertmafbbE1P1Egfp
TagsEGFPExpressionBacterialAvailable SinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cHAIAA7-spo5DSR
Plasmid#49873Purposefor Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsIAA17 degron tagAvailable SinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZF-BCB-GCN
Plasmid#127476Purposecoding sequence for synthetic zinc finger ZF-BCB-GCNDepositorInsertZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
UseCell-free transcription–translationTagsGCN4 leucine zipperPromoterT7Available SinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSmaug-V5His6_C
Plasmid#146040PurposeInsect Expression of DmSmaugDepositorInsertDmSmaug (smg Fly)
ExpressionInsectMutation10 silent mutations and a deletion of the AA 961-…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only