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pENTR4_FUS_G2A_R521H

Plasmid

#26370

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Use

Entry vector

Mutation

R521H

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 AAGY

Plasmid

#27004

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

P181A P182A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 LC WT

Plasmid

#26996

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 133-425

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pM2_PLLaco1R1CI*_PLTetO1R1CI*

Plasmid

#250895

Purpose

Cassette 1: Mutated cI protein expressed under PL TetO1 promoter; Cassette 2: Mutated cI protein expressed under PL LacO1 promoter; part of BNeu AS10; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

Mutated cI
Mutated cI

Use

Synthetic Biology

Mutation

cI* = Frame shifted cI protein

Promoter

PL LacO1 and PL TetO1

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-8xGTIIC-mScarlet-NLS-PEST

Plasmid

#249724

Purpose

Nuclear-localized fluorescent reporter of YAP/TAZ activity driven by promoter with TEAD binding sites

Depositor

Tobias Meyer

Article

Ferrick et al bioRxiv [Preprint]. 2024 Nov 4:2024.10.1

Insert

YAP-TEAD responsive promoter (YAP1 Human)

Use

Lentiviral

Tags

mScarlet3-NLS-PEST

Expression

Mammalian

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLLacO1R3CIw_PLux*R1cI*

Plasmid

#250891

Purpose

Frame shifted cI protein expressed under PLux* promoter; wild type cI expressed under PLLacO1 promoter; component of BNeuAS6; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

Mutated cI
Wild type cI

Use

Synthetic Biology

Mutation

cI* = Frame shifted cI protein; PLux* = Point mut…

Promoter

PLLacO1 and PLux*

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLLacO1R3CIw_PLTetO1R2CIw

Plasmid

#250893

Purpose

Cassette 1: Wild type cI protein expressed under PL TetO1, regulated by RBS 'R2'; Cassette 2: Wild type cI protein expressed under PL LacO1 and regulated b RBS 'R3'; part of Bneu AS7; p15A origin

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

Wild type cI
Wild type cI

Use

Synthetic Biology

Promoter

PL LacO1 and PL TetO1

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pNS3_Ptrc::luxR _Plux::Cdv3/GFP

Plasmid

#239009

Purpose

Cell elongation, under LuxR control, activation by 3OC6-HSL and 3OC12-HSL

Depositor

Daniel Ducat

Article

Kokarakis et al Metab Eng. 2025 Nov;92:76-89. doi: 10.10

Inserts

Transcriptional activator luxR
DivIVA-like cell division regulating protein

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Promoter

PluxI and Ptrc

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/Integrin-b5-FL-SGx3-Halo9

Plasmid

#249166

Purpose

Mammalian expression vector of human ITGB5 fused with Halo9-tag

Depositor

Pakorn Kanchanawong

Article

Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi:

Insert

Integrin beta-5 (ITGB5 Human)

Tags

Halo9

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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mIFP-Talin1(1–2493)-pdDronpa1.2

Plasmid

#249170

Purpose

Mammalian expression vector of opto-dimerizable talin1

Depositor

Pakorn Kanchanawong

Article

Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi:

Insert

Talin1 (TLN1 Human)

Tags

mIFP and pdDronpa1.2

Expression

Mammalian

Mutation

Deleted amino acids 2494–2541

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pdCas12f1_CBE-LVA

Plasmid

#220994

Purpose

Prokaryotic and constitutive expression of AsCas12f1/APOBEC1-LVA fusion

Depositor

John van der Oost

Article

Swartjes et al iScience. 2025 Nov 12;28(12):114033. doi

Insert

Acidibacillus sulfuroxidans Cas12f1-CBE

Use

CRISPR

Tags

LVA

Expression

Bacterial

Mutation

D225A

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pdCas12f1Cas_ABE-LVA

Plasmid

#220995

Purpose

Prokaryotic and constitutive expression of AsCas12f1/TadA8e-LVA fusion

Depositor

John van der Oost

Article

Swartjes et al iScience. 2025 Nov 12;28(12):114033. doi

Insert

Acidibacillus sulfuroxidans Cas12f1-ABE

Use

CRISPR

Tags

LVA

Expression

Bacterial

Mutation

D225A

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_TIMM23_ER-dAPEX-BFP2

Plasmid

#236734

Purpose

Dual sgRNA targeting TIMM23 expressing dAPEX-BFP targeting to ER

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TIMM23 (TIMM23 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKH032

Plasmid

#224548

Purpose

2A-3xNLS-2xClover mouse Car2 C-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

2xClover

Use

Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKH040

Plasmid

#224549

Purpose

2A-3xNLS-tdTomato mouse Car2 C-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

tdTomato

Use

CRISPR and Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SNAPf-Sec61b

Plasmid

#247295

Purpose

Mammalian expression of the SNAPf-tagged ER reporter SEC61b; to label ER membranes.

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

SNAPf-Sec61b (SEC61B Human)

Tags

SnapTag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1838

Plasmid

#239792

Purpose

pAAV-CMV-SNCAintron1-AlphaSyn[A53T]-WPRE-SV40pA (PD-INDUCER)

Depositor

Boris Kantor

Article

Kantor et al Mol Ther. 2018 Nov 7;26(11):2638-2649. d

Insert

CMV/SNCAintron1-AlphaSyn[A53T]-WPRE-SV40pA

Use

AAV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1575

Plasmid

#239793

Purpose

pLV-U6-[empty]-EFSNC-dSaCas9-[empty linker]-P2A-Puro-WPRE

Depositor

Boris Kantor

Article

Kantor et al Mol Ther. 2018 Nov 7;26(11):2638-2649. d

Insert

pLV-U6-[empty]-EFSNC-dSaCas9-[empty linker]-P2A-Puro-WPRE

Use

Lentiviral

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_H1-g3

Plasmid

#247169

Purpose

U6 driven gRNA expression of H1-g3 for targeting Dn29 attH1

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

H1-g3

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-CMV-DIO-MTS-roGFP-WPRE

Plasmid

#245375

Purpose

Cre-dependent expression of a green fluorescent protein biosensor to determine mitochondrial cellular reduction-oxidation (redox) states.

Depositor

D. James Surmeier

Article

Tubert et al Neurobiol Dis. 2023 Nov;188:106328. doi:

Insert

roGFP

Use

AAV

Tags

mitochondrial-targeting sequence of the S. pombe …

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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