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p415 TEF roGFP2‐Tsa2ΔCPΔCR

Plasmid

#83239

Purpose

The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

Insert

roGFP2-Tsa2dCpdCr

Tags

roGFP2 fusion with Tsa2dCpdCr

Expression

Yeast

Promoter

TEF

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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EYFP-JAB1

Plasmid

#111213

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Orel et al J Cell Mol Med. 2010 Jun;14(6B):1555-68.

Insert

CSN5 (COPS5 Human)

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-pEF1a-tTAc

Plasmid

#172123

Purpose

Constitutive expression of InteinC-tTAC

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

InteinC-tTAc

Use

AAV

Promoter

EF1a

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST12.2 DYRK2 WT

Plasmid

#20005

Depositor

Anjana Rao

Article

Gwack et al Nature. 2006 Jun 1. 441(7093):646-50.

Insert

DYRK2 (DYRK2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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tdTomato-Gamma-Tubulin-17

Plasmid

#58099

Purpose

Localization: Centrosomes, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
E1b-GFP-Tol2-Gateway

Plasmid

#37846

Depositor

Nadav Ahituv

Article

Birnbaum et al Genome Res. 2012 Jun;22(6):1059-68. Epub

Type

Empty backbone

Use

Zebrafish enhancer assay vector

Promoter

E1b

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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alpha-0 CFP in pcDNA3.1

Plasmid

#36396

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein alpha 0 (Gnao1 Rat)

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted into the KpnI-SpeI sites engineered …

Promoter

cmv

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pX330_DRS-1 sgRNA / hSpCas9

Plasmid

#172841

Purpose

Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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piggybac-Syn-mScn8a-3xHA

Plasmid

#182555

Purpose

Expresses Nav1.6 tagged with 3xHA

Depositor

Zhe J. Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Insert

Scn8a (Scn8a Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

Syn

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-hTel2

Plasmid

#30214

Depositor

Noboru Mizushima

Article

Kaizuka et al J Biol Chem. 2010 Jun 25. 285(26):20109-

Insert

telomere maintenance 2 (TELO2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only
pTRIPZ shIFNAR

Plasmid

#127699

Purpose

Doxycyclin inducible shRNA knockdown of mouse IFNAR gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shIFNAR1 Mmu (Ifnar1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG mEmerald-LAMP1

Plasmid

#168513

Purpose

Expression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes

Depositor

Franck Polleux

Article

Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

Insert

LAMP1 (Lamp1 Rat)

Tags

mEmerald

Expression

Mammalian

Promoter

CAG

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IP APEX-PEX26

Plasmid

#224562

Purpose

expresses APEX fused to PEX26 peroxisomal localization sequence in mammalian cells

Depositor

William Kaelin, Gregory Wyant

Article

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

PEX26 (PEX26 Human)

Use

Retroviral

Tags

APEX

Expression

Mammalian

Mutation

PEX26 peroxisomal targeting sequence

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-mOC-STAMP-GFP

Plasmid

#73579

Purpose

Lentivirus expression of mOC-STAMP

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Insert

OC-STAMP (Ocstamp Mouse)

Use

Lentiviral

Tags

GFP

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pSPAsp-hp

Plasmid

#48122

Purpose

Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

Insert

predicted optimal signal peptide for Bacillus megaterium protein secretion

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG_EGFP-GFE3

Plasmid

#79869

Purpose

Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-GFE3

Use

Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Promoter

Beta-actin

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B1

Plasmid

#172842

Purpose

CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mEGFP

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Clathrin-15

Plasmid

#58005

Purpose

Localization: Clathrin Vesicles, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Clathrin (CLTB Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CaMKII-GAL4-iLight-VP16

Plasmid

#170274

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16

Use

AAV

Mutation

See Comments

Promoter

CaMKII

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6-DCX-EGFP

Plasmid

#80599

Purpose

pLenti6 destination vector encoding C-terminally EGFP-tagged human doublecortin (DCX; GenBank: NM_178152)

Depositor

Torsten Wittmann

Article

Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

Insert

Doublecortin (DCX Human)

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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