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pFp10

Plasmid

#170460

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Publication

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

none

Expression

Insect

Promoter

dHsp70

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1DA

Plasmid

#35028

Depositor

Sheila Stewart

Publication

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1DA (FEN1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

D181A--NUCLEASE DEAD POINT MUTANT

Promoter

CMV

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAAV-Syn-SomaGCaMP6f1

Plasmid

#158755

Purpose

Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.

Depositor

Edward Boyden

Publication

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP6f1

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINHuVH

Plasmid

#234057

Purpose

Expression of human antibody Fd heavy chains - contains huIgG1 CH 1 sequence

Depositor

Ray Owens

Publication

Zhou et al Sci Adv. 2026 Jun 5;12(23):eaee8217. doi

Insert

Human CH1 heavy chain fragment (Fd)

Expression

Bacterial, Insect, and Mamm…

Available since

June 8, 2026

Availability

Academic Institutions and Nonprofits only

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pBOB-Gpr45-161-3xFlag

Plasmid

#245993

Purpose

Express 3xFlag C-terminal tagged mouse GPR45-N and GPR161-C fusion protein transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Publication

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45-GPR161161 fusion (Gpr161, Gpr45 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

aa 1 to 327 of Gpr45; aa 357 to 558 of Gpr161

Promoter

CMV

Available since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-Gpr45-Del1&2-3xFlag

Plasmid

#245981

Purpose

Express 3xFlag C-terminal tagged mouse GPR45-Del1&2 mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Publication

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gpr45 (Gpr45 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Del1&2, deletion of R223-D237 and R247-D259

Promoter

CMV

Available since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Nav1.6(K1425TAG)-HA

Plasmid

#239763

Purpose

Expresses mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Scn8a (Scn8a Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

K1425TAG

Promoter

CMV

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Nav1.6(K1546TAG)-HA

Plasmid

#239764

Purpose

Expresses mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Scn8a (Scn8a Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

K1546TAG

Promoter

CMV

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C)-P2A-eGFP

Plasmid

#239769

Purpose

Expresses independantly TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1425 and eGFP in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Scn8a (Scn8a Mouse)

Expression

Mammalian

Mutation

K1435TAG, Y371C

Promoter

CMV

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K519TAG)-HA

Plasmid

#239775

Purpose

Expresses rat NF186 with a TAG codon at position 519 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K519TAG

Promoter

CMV

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K534TAG)-HA

Plasmid

#239776

Purpose

Expresses rat NF186 with a TAG codon at position 534 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K534TAG

Promoter

CMV

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K571TAG)-HA

Plasmid

#239777

Purpose

Expresses rat NF186 with a TAG codon at position 571 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K571TAG

Promoter

CMV

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K604TAG)-HA

Plasmid

#239778

Purpose

Expresses rat NF186 with a TAG codon at position 604 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K604TAG

Promoter

CMV

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K809TAG)-HA

Plasmid

#239780

Purpose

Expresses rat NF186 with a TAG codon at position 809 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K809TAG

Promoter

CMV

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K519TAG)-HA

Plasmid

#239782

Purpose

Expresses rat NF186 with a TAG codon at position 519 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K519TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K534TAG)-HA

Plasmid

#239783

Purpose

Expresses rat NF186 with a TAG codon at position 534 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K534TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K571TAG)-HA

Plasmid

#239784

Purpose

Expresses rat NF186 with a TAG codon at position 571 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K571TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K604TAG)-HA

Plasmid

#239785

Purpose

Expresses rat NF186 with a TAG codon at position 604 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K604TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K680TAG)-HA

Plasmid

#239786

Purpose

Expresses rat NF186 with a TAG codon at position 680 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K680TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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hNSE-NF186(K809TAG)-HA

Plasmid

#239787

Purpose

Expresses rat NF186 with a TAG codon at position 809 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K809TAG

Promoter

hNSE (obtained from Addgene plasmid #11606)

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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