We narrowed to 8,732 results for: Set
-
Plasmid#153210PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGK111
Plasmid#228126PurposeExpress node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cellsDepositorInsertRetron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (SNP + PAM recode) in human cells
ExpressionMammalianPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGD335
Plasmid#219499PurposepAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoterDepositorInsertEco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
ExpressionBacterialPromoterpBADAvailable SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAT71
Plasmid#204305PurposeExpresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.DepositorInsertie1-DsRed
UseCRISPRPromoterie1Available SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIFV
Plasmid#111085PurposepNIFV is a vector plasmid with mutations in the cis-acting region, to prevent correction of the integrase by recombination when used with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectorsDepositorInsertempty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region
UseDeleted foamy virusMutationat nt 2334 A changed to T, at nt 2495 A changed t…PromoterCMVAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBK.079
Plasmid#187218PurposeExpresses Eco1 RT and Cas1 + Cas2DepositorInsertsRetron Eco1 Reverse Transcriptase
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterJ23115 and T7/lacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGoldenGreenGate-M (pGGG-M)
Plasmid#165422PurposeA Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).TypeEmpty backboneUseSynthetic Biology; Level 2 golden gate (moclo) du…PromoterContain GG cassette and MCS with LacZ for blue/wh…Available SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre stuffer v3
Plasmid#158030Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9DepositorTypeEmpty backboneExpressionMammalianAvailable SinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTS2355_Tier3(PB)-iRFP_PuroR
Plasmid#169654PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven iRFP670 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE
Plasmid#223287PurposeA complete OptoMBP cassette for dissolution of FUS-CHOP condensates.DepositorInsertmyc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS
UseSynthetic Biology; Piggybac transposase insertabl…ExpressionMammalianAvailable SinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Blast
Plasmid#227270PurposeEmpty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F2
Plasmid#184164PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP
Plasmid#158963PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed Non-FP cassette
Cas9-GFP
UseCRISPR and Cre/LoxAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOCC102
Plasmid#118883Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C proteaseDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-GFP-HYGRO
Plasmid#115144PurposeSafe harbor site 231 knock-in vector with GFP-HygromycinR expression cassetteDepositorInsertGFP
ExpressionMammalianPromoterEF1aAvailable SinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRECMYC-Ebu1
Plasmid#240713PurposepLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155DepositorInsertEbu1 RT, Eco1 ncRNA, MspRecT
ExpressionBacterialMutationWTAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-RFP-HYGRO
Plasmid#115145PurposeSafe harbor site 231 knock-in vector with RFP-HygromycinR expression cassetteDepositorInsertRFP
ExpressionMammalianPromoterEF1aAvailable SinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-loxP-STOP-loxP-Venus
Plasmid#170520PurposePlasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )DepositorInsertCAG-loxP-STOP-loxP-Venus
ExpressionMammalianPromoterCAGAvailable SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSP1673
Plasmid#65769PurposeBacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNADepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
UseCRISPRTagsNLSExpressionBacterialPromoterT7 (x2)Available SinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only