We narrowed to 14,099 results for: cas9

1. pKb-TnpB2

   Plasmid

   #215334

   Purpose

   ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site

   Depositor

   Kutubuddin Molla

   Article

   Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10

   Insert

   Rice codon optimized ISDra2 TnpB
   

   Tags

   Not applicable

   Expression

   Plant

   Promoter

   Rice ubiquitin promoter for TnpB and maize ubiqui…

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYLsgRNA-OsU6a

   Plasmid

   #66194

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   OsU6a

   Available Since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYLsgRNA-AtU6-1

   Plasmid

   #66202

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRIM28 gRNA (BRDN0001162487)

   Plasmid

   #76139

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM28

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM28 (TRIM28 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pECNUS4

   Plasmid

   #184887

   Purpose

   ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

   Depositor

   Eunyoung Chae

   Article

   Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   RPS5A

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. gh38

   Plasmid

   #106712

   Purpose

   expression of gRNA targeting STT3A

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   STT3A (STT3A Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYLsgRNA-AtU3b

   Plasmid

   #66198

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Promoter

   AtU3b

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRIM28 gRNA (BRDN0001146162)

   Plasmid

   #76142

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM28

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM28 (TRIM28 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. PTK2 gRNA (BRDN0001146703)

   Plasmid

   #75544

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PTK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PTK2 (PTK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid

    #139988

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PTK2 gRNA (BRDN0001148426)

    Plasmid

    #75545

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PTK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PTK2 (PTK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PTK2 gRNA (BRDN0001145734)

    Plasmid

    #75543

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PTK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PTK2 (PTK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRIB2 gRNA (BRDN0001144754)

    Plasmid

    #75602

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIB2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIB2 (TRIB2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRIB2 gRNA (BRDN0001148141)

    Plasmid

    #75603

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIB2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIB2 (TRIB2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-LoxP-PURO-LoxP-SNAP-TERT

    Plasmid

    #71390

    Purpose

    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Use

    Hr donor

    Tags

    FLAG-SNAP

    Promoter

    Endogenous TERT promotor

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. RNASEL gRNA (BRDN0001146030)

    Plasmid

    #77053

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. RNASEL gRNA (BRDN0001148937)

    Plasmid

    #77054

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKb-TnpB1

    Plasmid

    #215333

    Purpose

    ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site

    Depositor

    Kutubuddin Molla

    Article

    Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10

    Insert

    Rice codon optimized ISDra2 TnpB
    

    Tags

    Not applicable

    Expression

    Plant

    Promoter

    Rice ubiquitin promoter for TnpB and OsU3 promote…

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUFlip-floxed2A-mRFP-; gcry1:BFP -0

    Plasmid

    #173886

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; gcry1: BFP
    

    Use

    Synthetic Biology

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. non-targeting control gRNA (BRDN0001149198)

    Plasmid

    #80248

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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