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pBOB-Adcy3-HA

Plasmid

#245988

Purpose

Express HA C-terminal tagged wild-type mouse ADCY3 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Adcy3 (Adcy3 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pBOB-Adcy3-L278H-3xFlag

Plasmid

#245990

Purpose

Express 3xFlag C-terminal tagged mouse ADCY3-L278H mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Adcy3 (Adcy3 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

L278H

Promoter

CMV

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-FCRL3-3xFLAG-puro

Plasmid

#248155

Purpose

Lentiviral vector expressing human FCRL3 with 3xFLAG tags

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Tags

3x FLAG

Mutation

A 3xFLAG epitope tag is fused in frame to the C-t…

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(mem).iGlucoSnFR2

Plasmid

#244096

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).cpSFGFP.HaloTag

Plasmid

#244109

Purpose

Cytosolic expression of non-responsive circularly permuted Super Folder GFP

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

cpSFGFP

Use

AAV

Tags

HaloTag

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr201 RNF146 deltaWWE

Plasmid

#240331

Purpose

Entry vector for Gateway with RNF146 (WWE domain deletion)

Depositor

Junjie Chen

Article

Nie et al Mol Cell Proteomics. 2020 Dec;19(12):201

Insert

RNF146 (RNF146 Human)

Expression

Bacterial

Mutation

WWE domain deletion

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-EGFP-mPkm2

Plasmid

#242812

Purpose

Lentiviral vector expressing mouse Pkm2 tagged with an N-terminal EGFP

Depositor

Ronald Hart

Article

Sun et al Nat Commun. 2025 Oct 21;16(1):9312. doi:

Insert

pyruvate kinase, muscle (Pkm Mouse)

Use

Lentiviral

Tags

EGFP

Promoter

Ubiquitin C

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ER-GFP pLVX CMVpuro

Plasmid

#246912

Purpose

Lentiviral-based expression of EGFP with an ER retention signal sequence in mammalian cells

Depositor

William Kaelin

Article

Lampson et al Cell. 2024 Apr 25;187(9):2209-2223.e16.

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.mIRFP670nano3

Plasmid

#244107

Purpose

Bacterial expression of green glucose sensor with inert mIRFP670nano3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mIRFP670nano3

Tags

mIRFP670nano3

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr201 RNF146 deltaRING

Plasmid

#240332

Purpose

Entry vector for Gateway with RNF146 (RING domain deletion)

Depositor

Junjie Chen

Article

Nie et al Mol Cell Proteomics. 2020 Dec;19(12):201

Insert

RNF146 (RNF146 Human)

Expression

Bacterial

Mutation

RING domain deletion

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSB02

Plasmid

#230991

Purpose

B. dendrobatidis plasmid for integration of target sequence at URA3 locus, blasticidin S selection

Depositor

Lillian Fritz-Laylin

Article

Brody et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

BdURA3_LHA2-Hsbsr-ScADH1ter

Use

Bd gene targeting vector

Tags

bsr

Expression

Bacterial

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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EBFP2-Kindlin2-3KA

Plasmid

#242823

Purpose

Encodes human kindlin2-3KA mutant and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Kindlin2 (FERMT2 Human)

Expression

Mammalian

Mutation

K385A, K386A, K393A

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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EBFP2-K1K2

Plasmid

#242819

Purpose

Encodes human kindlin1 containing kindlin2 F3 domain and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Kindlin1 (Fermt1 Human)

Expression

Mammalian

Mutation

Kindlin1 chimera containing the F3 domain of kind…

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDonr 201_MGA

Plasmid

#240327

Purpose

Entry vector for Gateway with MGA

Depositor

Junjie Chen

Article

Nie et al Genes Dev. 2022 Sep 1;36(17-18):1016-103

Insert

MGA (MGA Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-Gnai3

Plasmid

#245997

Purpose

Express HA N-terminal tagged mouse Galphai3 transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnai3 (Gnai3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-GFP

Plasmid

#243759

Purpose

AAV plasmid expressing GFP under the CAG promoter.

Depositor

Andrew Arrant

Article

Nader et al Sci Rep. 2025 Jul 19;15(1):26217. doi: 1

Insert

Enhanced Green Fluorescent Protein

Use

AAV

Promoter

CAG

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-Adcy3-3xFlag

Plasmid

#245989

Purpose

Express 3xFlag C-terminal tagged wild-type mouse ADCY3 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Adcy3 (Adcy3 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-HA-Gnai1

Plasmid

#245996

Purpose

Express HA N-terminal tagged mouse Galphai1 transiently via transfection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gnai1 (Gnai1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.hSynap.(ER).iGlucoSnFR2.HaloTag

Plasmid

#244076

Purpose

ER targeted expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Use

AAV

Available Since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(mem).iGlucoSnFR2

Plasmid

#244088

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.GFAP.(cyto).iGlucoSnFR2

Plasmid

#244111

Purpose

Cytosolic expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pNoRBP.iGlucoSnFR2.HaloTag

Plasmid

#244105

Purpose

Bacterial expression of green glucose sensor with inert HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Tags

HaloTag

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAGFLEX.(cyto).iGlucoSnFR2

Plasmid

#244078

Purpose

Cytosolic expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAGFLEX.(mem).iGlucoSnFR2

Plasmid

#244081

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pNoRBP.iGlucoSnFR2.H348H.HaloTag

Plasmid

#244108

Purpose

Bacterial expression of green glucose sensor (high affinity) with inert HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Tags

HaloTag

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pNoRBP.iGlucoSnFR2.mRuby3

Plasmid

#244106

Purpose

Bacterial expression of green glucose sensor with inert mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Tags

mRuby3

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EBFP2-K2K1

Plasmid

#242820

Purpose

Encodes human kindlin2 containing kindlin1 F3 domain and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Kindlin2 (FERMT2 Human)

Expression

Mammalian

Mutation

Kindlin2 chimera containing the F3 domain of kind…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP-SFFV-tagBFP-2A-FLAG-coBICP0

Plasmid

#235543

Purpose

To express tagBFP-2A-FLAG-coBICP0 (co = codon-optimized)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

BICP0

Use

Lentiviral

Tags

FLAG

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-Nsp3(1-111)

Plasmid

#242947

Purpose

GFP and the Ubl1 domain of the SARS-CoV-2 Nsp3 protein (aa 1-111)

Depositor

Benjamin Schuster

Article

Favetta et al bioRxiv [Preprint]. 2025 Jun 10:2024.10.

Insert

GFP-Nsp3(1-111) (ORF1ab SARS-CoV-2)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Integrin β6β1-EGFP

Plasmid

#242829

Purpose

Encodes human integrin β6 chimera containing integrin β1 cytoplasmic domain and C-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Integrin subunit beta 6, transcript variant 1 (ITGB6 Human)

Expression

Mammalian

Mutation

Integrin β6 chimera containing the cytoplasmic ta…

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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