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pcDNA3.1 2xFLAG-2xSTREP-KEAP1 (human)

Plasmid

#236422

Purpose

transient overexpression of KEAP1 in mammalian cells

Depositor

Michele Pagano

Article

Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

Insert

KEAP1 (KEAP1 Human)

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP14-SARS-CoV

Plasmid

#236424

Purpose

bacterial protein expression of NSP14

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP14 (ORF1ab SARS-CoV)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP14-MERS-CoV

Plasmid

#236425

Purpose

bacterial protein expression of NSP14

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP14 (orf1ab MERS-CoV)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP10-SARS-CoV-2

Plasmid

#236426

Purpose

bacterial protein expression of NSP10

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP10 (ORF1ab SARS-CoV-2)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP10-SARS-CoV

Plasmid

#236427

Purpose

bacterial protein expression of NSP10

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP10 (ORF1ab SARS-CoV)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pET-30a-NSP10-MERS-CoV

Plasmid

#236428

Purpose

bacterial protein expression of NSP10

Depositor

Michele Pagano

Article

Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

Insert

NSP10 (orf1ab MERS-CoV)

Tags

6xHis

Expression

Bacterial

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-2xFLAG-2xSTREP-E2F1

Plasmid

#236433

Purpose

doxycycline inducible expression of E2F1 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F1 (E2F1 Human)

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-2xFLAG-2xSTREP-E2F2

Plasmid

#236434

Purpose

doxycycline inducible expression of E2F2 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F2 (E2F2 Human)

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-2xFLAG-2xSTREP-E2F3A

Plasmid

#236435

Purpose

doxycycline inducible expression of E2F3A in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F3A (E2f3 Mouse)

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 K-RAS 4A

Plasmid

#236451

Purpose

transient overexpression of K-RAS 4A in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

K-RAS 4A (KRAS Human)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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NLS-Cherry-PCNA-P2A-Venus-E2F1

Plasmid

#236446

Purpose

insect expression of E2F1 for protein purification

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F1 (E2F1 Human)

Expression

Insect

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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NLS-Cherry-PCNA-P2A-Venus-E2F2

Plasmid

#236447

Purpose

insect expression of E2F2 for protein purification

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F2 (E2F2 Human)

Expression

Insect

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 FLAG Fbxw7gamma

Plasmid

#236471

Purpose

transient overexpression of FBXW7gamma in mammalian cells

Depositor

Michele Pagano

Article

Busino et al Nat Cell Biol. 2012 Mar 4;14(4):375-85.

Insert

Fbxw7gamma (FBXW7 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Step-FLAG FBXO21

Plasmid

#236458

Purpose

transient overexpression of FBXO21 in mammalian cells

Depositor

Michele Pagano

Article

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

FBXO21 (FBXO21 Human)

Tags

Strep-FLAG

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Strep-FLAG KBP

Plasmid

#236459

Purpose

transient overexpression of KBP in mammalian cells

Depositor

Michele Pagano

Article

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

KBP (KIF1BP Human)

Tags

Strep-FLAG

Expression

Mammalian

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar2

Plasmid

#232739

Purpose

Expresses NADPH/NADP+ biosensor NAPstar2 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar2

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar4

Plasmid

#232741

Purpose

Expresses NADPH/NADP+ biosensor NAPstar4 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar4

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar6

Plasmid

#232742

Purpose

Expresses NADPH/NADP+ biosensor NAPstar6 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar6

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar7

Plasmid

#232744

Purpose

Expresses NADPH/NADP+ biosensor NAPstar7 in S. cerevisiae.

Depositor

Bruce Morgan

Article

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar7

Expression

Yeast

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S91, 262 ,331, 724A

Plasmid

#224738

Purpose

For expression of HA-tagged GTSE1 protein with S91, 262 ,331, 724A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Tags

HA

Expression

Mammalian

Mutation

Serine 91, 262 ,331, 724 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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