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pDARMO_CMVT_3xFLAG-HUWE1_H669Q

Plasmid

#187138

Purpose

mammalian cell expression of 3xFLAG-HUWE1 H669Q

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

H669Q

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_delta169-189

Plasmid

#187136

Purpose

mammalian cell expression of FLAG-HUWE1 delta169-189

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

delta169-189

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_delta169-189

Plasmid

#187135

Purpose

mammalian cell expression of 3xFLAG-HUWE1 delta169-189

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

delta169-189

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDARMO_CMVT_3xFLAG-HUWE1_delta3896-3951

Plasmid

#187133

Purpose

mammalian cell expression of 3xFLAG-HUWE1 delta3896-3951

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

delta3896-3951

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_I3969A/F3982A

Plasmid

#187129

Purpose

mammalian cell expression of 3xFLAG-HUWE1 I3969A/F3982A

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

I3969A/F3982A

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC8-Mcl1_deltaTM

Plasmid

#187118

Purpose

insect cell expression of Mcl1-deltaTM

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

MCL-1 (BCL2 Human)

Use

Tags

Strep

Expression

Insect

Mutation

delta328-350

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-DDIT4_delta8

Plasmid

#187117

Purpose

insect cell expression of DDIT4-delta1-8

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

DDIT4 (DDIT4 Human)

Use

Tags

Strep

Expression

Insect

Mutation

delta1-8

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-DDIT4

Plasmid

#187116

Purpose

insect cell expression of DDIT4

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

DDIT4 (DDIT4 Human)

Use

Tags

Strep

Expression

Insect

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-HUWE1-HECTonly

Plasmid

#187119

Purpose

insect cell expression of HUWE1 HECT domain

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

Strep

Expression

Insect

Mutation

delta1-3992

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBA/UIM/UBM-mut

Plasmid

#187153

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBA/UIM-mut

Plasmid

#187151

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

M1328A/F1330A/A1376G/S1382A

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-HUWE1_UBM-mut

Plasmid

#187149

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

L2976A/P2977A

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-HUWE1_UBA-mut

Plasmid

#187145

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

M1328A/F1330A

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-HUWE1_BH3-4E

Plasmid

#187143

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

V1976E/V1780E/L1983E/M1987E

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-HUWE1_R4187C

Plasmid

#187141

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

R4187C

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-HUWE1_F3194S

Plasmid

#187139

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

F3194S

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-HUWE1_H669Q

Plasmid

#187137

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

H669Q

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-TagBFP-P2A-Pre-SPHERE-Rncp-iGluSnFR1

Plasmid

#187093

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-Rncp-iGluSnFR1

Use

Tags

TagBFP-P2A

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-iRFP-P2A-Post-SPHERE-Rncp-iGluSnFR1

Plasmid

#187094

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Post-SPHERE-Rncp-iGluSnFR1

Use

Tags

iRFP670-P2A

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-mCherry-P2A-Pre-SPHERE-sfmTurquoise2-iGluSnFR

Plasmid

#187095

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-sfmTurquoise2-iGluSnFR

Use

Tags

mCherry-P2A

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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