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LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode

Plasmid

#170236

Purpose

Barcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CHUK (CHUK Human)

Use

Lentiviral

Tags

V5

Mutation

S176E, S180E

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-eArchT 3.0-EYFP

Plasmid

#35513

Purpose

Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eArchT 3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-FLEX-rc [SomArchon-GFP]

Plasmid

#153533

Purpose

AAV-mediated expression of SomArchon-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon-GFP

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Promoter

Syn

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode

Plasmid

#170220

Purpose

Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

CDK1 (CDK1 Human)

Use

Lentiviral

Mutation

T14A, Y15F

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA(MS2)-puro-barcode

Plasmid

#89493

Purpose

Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

Depositor

Gary Hon

Article

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

sgRNA (with MS2 loop)

Use

Lentiviral

Promoter

U6

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFAST-BAC BAF57/SMARCE1-His

Plasmid

#177861

Purpose

Transfer vector to generate recombinant baculovirus expressing BAF57/SMARCE1-His protein

Depositor

Yoshihiro Izumiya

Article

Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

Insert

SMARCE1 (SMARCE1 Human)

Tags

His tag at C-terminus with GGGGS linker

Expression

Insect

Promoter

polyhedrin

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FDIO-ArchT-GFP

Plasmid

#124640

Purpose

AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

Depositor

David Dupret

Article

Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

Insert

ArchT

Use

AAV; Flp/frt

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-ArchT-GFP (PV2527)

Plasmid

#99039

Purpose

AAV-mediated expression of ArchT under the CamKII promoter

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

ArchT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CamKII

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-ArchT-GFP

Plasmid

#58851

Purpose

AAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) manner

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

Plasmid

#114111

Purpose

fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

Insert

Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-eArch 3.0-EYFP

Plasmid

#35514

Purpose

Lentiviral expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eArch3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pNH11 (pmyo-2::Arch(D95N)::2xMycTag)

Plasmid

#130275

Purpose

Expresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

Arch(D95N)

Tags

2x Myc-tag

Expression

Worm

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pPB CAG SMARCA4 T910M-Venus

Plasmid

#153951

Purpose

piggyBac transposon vector with CAG promoter expressing SMARCA4 (T910M mutant)-Venus fusion protein

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

SMARCA4 (SMARCA4 Human)

Tags

Venus

Expression

Mammalian

Mutation

T910M

Promoter

CAG

Available Since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

Plasmid

#170240

Purpose

Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

S218D, S222D

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode

Plasmid

#170228

Purpose

Barcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

Map2k7-MAPK9 fusion (MAPK9, Map2k7 Mouse, Human)

Use

Lentiviral

Tags

FLAG

Mutation

Fusion

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO Myc ArC Long WT

Plasmid

#59809

Purpose

Allows the integration of Myc ArC Long in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora C (AURKC Human)

Tags

Myc

Expression

Mammalian

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSF143-wArchon2-KGC-EGFP-ER2

Plasmid

#108430

Purpose

Codon-optimized Archon2 fluorescent voltage reporter in C.elegans expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

wArchon2-KGC-EGFP-ER2

Expression

Worm

Promoter

flp-18

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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library backbone 3'LTR gRNA and barcode

Plasmid

#127397

Purpose

Digestion by BstXI and BamHI to release the backbone

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 28;11

Type

Empty backbone

Use

Lentiviral

Tags

Puro-mCherry

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α1.1-FLEX-rc [SomArchon-GFP]

Plasmid

#153532

Purpose

AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon-GFP

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Promoter

EF1α1.1

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO Myc ArC K72R

Plasmid

#59811

Purpose

Allows the integration of Myc ArC K72R in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora C (AURKC Human)

Tags

Myc

Expression

Mammalian

Mutation

K72R, kinase mutant

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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