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Plasmid#123365PurposeExpression of full-length mouse GSDMD in E. coliDepositorInsertGSDMD (Gsdmd Mouse)
UseTagsHis6-MBPExpressionBacterialMutationPromoterT7Available sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
mouse Oct4-GFP GOF18 transgenic reporter
Plasmid#60527PurposeBAC recombineering generated construct, used as a reporter for Oct4 expression (containes both DE and PE elements)DepositorInsertGFPpA
UseTagsExpressionMammalianMutationPromoterOct4Available sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
FOR human OCT4 TALEN
Plasmid#52412PurposeTALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)DepositorInsertHD NG NN NN NN HD NG HD NG HD HD HD NI NG
UseTALENTagsExpressionMutationPromoterCAGGSAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc3-Mdm2 p60
Plasmid#52059Purposeexpression of truncated Mdm2 in mammalian cellsDepositorInsertMdm2 p60 (truncated) (MDM2 Human)
UseTagsmyc3ExpressionMammalianMutationp60 (truncated at D367, DVDP motif)PromoterCMVAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
FUW-Flag-Mbd3
Plasmid#52371PurposeLentiviral expression vector for constitutive expression of double Flag tagged Mbd3DepositorInsertMbd3 (Mbd3 Mouse)
UseLentiviralTagsflagExpressionMammalianMutationPromoterUbAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
REV human OCT4 TALEN
Plasmid#52413PurposeTALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)DepositorInsertHD HD HD HD HD NI NG NG HD HD NG NI NN NI NI NN NN
UseTALENTagsExpressionMutationPromoterCAGGSAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
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RANLS-LEHD-BNES
Plasmid#60973PurposeExpresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).DepositorInsertddRFP A and ddRFP B
UseTagsCaspase9 substrate sequence (LEHD) is after NLS s…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-CAGGS-ERAS
Plasmid#52416PurposeLentiviral vector encoding constitutive expression of human ERAS cDNADepositorInsertERAS (ERAS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-lox-ERAS
Plasmid#52417PurposeDoxycycline inducible lentiviral vector of human ERAS cDNA with excisable insertDepositorInsertERAS (ERAS Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNTK human MBD3 PGK-Neo-pA flox
Plasmid#52362PurposeTargeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles.DepositorInsertMBD3 (MBD3 Human)
UseTargetingTagsExpressionMutationPromoterAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_huCAD_ICADL (TevS)
Plasmid#100098Purposedual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequencesDepositorUseTagsExpressionBacterialMutationtwo caspase cleavage sites (DETD-117 and DAVD-224…PromoterT7Available sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
PMXS-GOT1
Plasmid#72872PurposeThe retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.DepositorInsertGOT1 (GOT1 Human)
UseRetroviralTagsExpressionMammalianMutationsgRNA resistant human GOT1, 7 silent mutations c1…PromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
OCT4-GFP-2A-PURO transgenic reporter
Plasmid#52379PurposeBAC recombineering generated construct, used as a reporter for OCT4 expressionDepositorInsertsGFP-2A-puro
OCT4
UseTagsExpressionMammalianMutationPromoterOCT4Available sinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
FUW-Flag-Mbd3 delta1-70
Plasmid#52372PurposeLentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutantDepositorInsertMbd3 (Mbd3 Mouse)
UseLentiviralTagsflagExpressionMammalianMutationdeleted amino acids 1-70PromoterUbiquitinAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
FUW-Flag-Mbd3 delta 25-83
Plasmid#52373PurposeLentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutantDepositorInsertMbd3 (Mbd3 Mouse)
UseLentiviralTagsflagExpressionMammalianMutationamino acids 25-83 deletedPromoterUbiquitinAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
FUW-Flag-Mbd3 delta49-63
Plasmid#52374PurposeLentiviral expression vector for constitutive expression of double Flag tagged Mbd3 mutantDepositorInsertMbd3 (Mbd3 Mouse)
UseLentiviralTagsflagExpressionMammalianMutationdeleted amino acids 49-63PromoterUbiquitinAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBRPy CAGGS-Mbd3-IRES-PURO
Plasmid#52376PurposeMammalian expression vector for constitutive expression of Mbd3DepositorInsertMbd3 (Mbd3 Mouse)
UseTags2xFlag (DYKDDK)ExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-human OCT4 PE-SV40-Luc
Plasmid#52415PurposeLuciferase reporter plasmid for PE OCT4 enhancer activityDepositorInsertProximal element of hOCT4 promoter (POU5F1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO
Plasmid#52419PurposeConstitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.DepositorInsertERAS (ERAS Human)
UseTagsExpressionMutationPromoterCAGGSAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only