Addgene

We narrowed to 23,369 results for: CHI;

Showing: 681 - 700 of 23369 results

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pET28(a)-hFX-HA

Plasmid

#14991

Depositor

Michael Ristow

Article

Dhe-Paganon et al J Biol Chem. 2000 Oct 6. 275(40):30753-6

Insert

human frataxin cDNA (FXN Human)

Use

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pBlue-hFX(AA88)-HA

Plasmid

#15402

Depositor

Michael Ristow

Article

Dhe-Paganon et al J Biol Chem. 2000 Oct 6. 275(40):30753-6

Insert

human frataxin cDNA (FXN Human)

Use

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
psgRNA Library

Pooled Library

#115927

Purpose

Genome-wide knockdown of E. coli genes

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Expression

Bacterial

Species

Escherichia coli

Use

CRISPR

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only
T7-VEE-GFP

Plasmid

#58977

Purpose

expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

EGFP

Use

Venezuelan equine encephalitis (vee) virus rna re…

Tags

Expression

Mammalian

Mutation

Promoter

26S subgenomic promoter

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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SP6-VEE-GFP

Plasmid

#58976

Purpose

expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

EGFP

Use

Venezuelan equine encephalitis (vee) virus rna re…

Tags

Expression

Mammalian

Mutation

Promoter

26S subgenomic promoter

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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p3HA-hMIB2

Plasmid

#33312

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

MIB2 (MIB2 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 26, 2012

Availability

Academic Institutions and Nonprofits only
PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP

Plasmid

#172670

Purpose

For expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

SDHB pegRNA and SDHB_nick-sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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PGL3-U6-ALDOB_pegRNA-Csy4RS-nick_sgRNA-EGFP

Plasmid

#172668

Purpose

For expression of transcript containing pegRNA and nick-sgRNA targeting human ALDOB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

ALDOB pegRNA and ALDOB_nick-sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP

Plasmid

#172669

Purpose

For expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

RNF2 pegRNA and RNF2_nick-sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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psgRNA_EcoWG1

Pooled Library

#131625

Purpose

Improved genome-wide knockdown of E. coli genes

Depositor

David Bikard

Article

Calvo-Villamanan et al Nucleic Acids Res. 2020 Apr 30. pii: 582

Expression

Bacterial

Species

Escherichia coli

Use

CRISPR

Available since

May 12, 2020

Availability

Academic Institutions and Nonprofits only
p3HA-hMIB1

Plasmid

#33317

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

MIB1 (MIB1 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl-2-s2193

Plasmid

#166750

Purpose

Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2 in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-2 (BCL2 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

Human ferritin

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-tBid-pEGFP-C1

Plasmid

#166736

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

tBid (Bid Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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p3F-Strep-mMALT1

Plasmid

#33315

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

Malt1 (Malt1 Mouse)

Use

Tags

3xFLAG and StrepOne

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
plenti6.3/hCx43-stop

Plasmid

#27383

Depositor

Robin Shaw

Article

Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

Insert

Gja1 (GJA1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only
Venus-Bad-pEGFP-C1

Plasmid

#166733

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bad (BAD Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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p3F-Strep-mBCL10

Plasmid

#33314

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

Bcl10 (Bcl10 Mouse)

Use

Tags

3xFLAG and StrepOne

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
pTRE-TIGHT-Cx43-eYFP

Plasmid

#31807

Depositor

Robin Shaw

Article

Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

Insert

Cx43 (GJA1 Human)

Use

Tags

eYFP

Expression

Mammalian

Mutation

Promoter

P-TIGHT

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
Venus-BimEL-pEGFP-C1

Plasmid

#166735

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, BimEL

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

BimEL (Bcl2l11 Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl2-pEGFP-C1

Plasmid

#177416

Purpose

To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-2 (BCL2 Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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