We narrowed to 1,058 results for: IM

1. pY010 (pcDNA3.1-hAsCpf1)

   Plasmid

   #69982

   Purpose

   Expresses humanized AsCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hAsCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY004 (pcDNA3.1-hFnCpf1)

   Plasmid

   #69976

   Purpose

   Expresses humanized FnCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hFnCpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC003 - LshC2C2 locus into pACYC184 for spacer cloning

   Plasmid

   #79152

   Purpose

   LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

   Insert

   LshC2c2 locus
   

   Use

   Tags

   Expression

   Mutation

   *

   Promoter

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP

   Plasmid

   #90008

   Purpose

   Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   NBirA*-linker-FKBP
   FRB-linker-CBirA*
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir

   Plasmid

   #90004

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   FKBP-linker-Myc-NBirA*
   CBirA*-linker-FRB
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSF3-Flag-CBir-FRB_Myc-NBir-FKBP

   Plasmid

   #90003

   Purpose

   Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   NBirA*-linker-FKBP
   CBirA*-linker-FRB
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir

   Plasmid

   #90009

   Purpose

   Split-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBP

   Depositor

   Julien Béthune

   Article

   Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

   Inserts

   FKBP-linker-Myc-NBirA*
   FRB-linker-CBirA*
   

   Use

   Retroviral; Flp/frt

   Tags

   FLAG and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pC4-RhE-FRB-Fis1

   Plasmid

   #68056

   Purpose

   express Fis1 tail-anchored FRB for heterodimerization with FKBP in mammalian cells

   Depositor

   Richard Youle

   Article

   Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

   Insert

   Fis1 (FIS1 Human)
   

   Use

   Tags

   FRB

   Expression

   Mammalian

   Mutation

   Fis1 tail (aa 93-152)

   Promoter

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMX IkB alpha M

   Plasmid

   #12329

   Depositor

   Inder Verma

   Article

   Van Antwerp et al Science. 1996 Nov 1. 274(5288):787-9.

   Insert

   IkB alpha M (Nfkbia Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Dominant-negative mutant. Many signal transductio…

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pY011 (pcDNA3.1-hLb2Cpf1)

    Plasmid

    #69983

    Purpose

    Expresses humanized Lb2Cpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLb2Cpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLAC

    Plasmid

    #45072

    Purpose

    lacritin-intein fusion plasmid coding for intact mature lacritin without signal peptide

    Depositor

    Gordon Laurie

    Article

    Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

    Insert

    Lacritin (LACRT Human)
    

    Use

    Protein expression vector

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. mILK-EFGP

    Plasmid

    #176896

    Purpose

    mouse ILK cloned into pEGFP-N1

    Depositor

    Sara Wickström

    Article

    Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    Integrin-linked kinase (Ilk Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pY014 (pcDNA3.1-hMbCpf1)

    Plasmid

    #69986

    Purpose

    Expresses humanized MbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hMbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKG139

    Plasmid

    #69755

    Purpose

    Retroviral expression of CENP-C

    Depositor

    Iain Cheeseman

    Article

    Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

    Insert

    CENP-C (CENPC Human)
    

    Use

    Retroviral

    Tags

    mCherry-TEV-S

    Expression

    Mammalian

    Mutation

    L341F, N570D relative to NP_001803.2 (numbering b…

    Promoter

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pY018 (pcDNA3.1-hPdCpf1)

    Plasmid

    #69990

    Purpose

    Expresses humanized PdCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPdCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKC158

    Plasmid

    #134242

    Purpose

    CENP-A Halo expression construct

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    CENP-A-Halo tagging construct (CENPA Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pY012 (pcDNA3.1-hCMtCpf1)

    Plasmid

    #69984

    Purpose

    Expresses humanized CMtCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hCMtCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pY017 (pcDNA3.1-hPcCpf1)

    Plasmid

    #69989

    Purpose

    Expresses humanized PcCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPcCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETa(+) HpCsd3 (42-403)

    Plasmid

    #71285

    Purpose

    Expresses Csd3 from Helicobacter pylori

    Depositor

    SeWon Suh

    Article

    An et al Acta Crystallogr D Biol Crystallogr. 201

    Insert

    HP0506 (HP0506 )
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

20. mCer-Vamp2(qv/fw)

    Plasmid

    #53238

    Purpose

    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Mutated Q76 to V; F77 to W.

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only