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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,164 results for: PAM

Showing: 681 - 700 of 2164 results

  1. pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139096
    Purpose
    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9 3.7
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139096-plasmid-map-sequence-id-273894

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  3. Cas12Max

    Plasmid
    #195334
    Purpose
    vector for encoding a human codon-optimized Cas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized Cas12Max
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R
    Promoter
    CBh, CMV, hU6
    Available Since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195334-plasmid-map-sequence-id-383108

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  6. xCas12i

    Plasmid
    #195333
    Purpose
    vector for encoding a human codon-optimized xCas12i driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized xCas12i
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CBh, CMV, hU6
    Available Since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195333-plasmid-map-sequence-id-383109

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  14. pUC19-POLBHR-eGFP

    Plasmid
    #176064
    Purpose
    Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    EGFP-PolB (POLB Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    pUC with endogenous SapI mutated out, PAM mutatio…
    Promoter
    N/A
    Available Since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    176064-plasmid-map-sequence-id-352734

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  15. Phage-PGK-GFP-T(WT)

    Plasmid
    #181736
    Purpose
    for lentiviral expression of brachyury ORF in mammalian cells
    Depositor
    Charles Lin
    Insert
    T (brachyury)
    Use
    Lentiviral
    Mutation
    a silent mutation in the PAM site of an sgRNAt ta…
    Available Since
    Aug. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    181736-plasmid-map-sequence-id-357223

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  16. pCas9CR5-NG

    Plasmid
    #120228
    Purpose
    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120228-plasmid-map-sequence-id-233263

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  17. pCas9CR4-NG

    Plasmid
    #120225
    Purpose
    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.
    Depositor
    Christopher Reisch
    Insert
    SpCas9-NG
    Use
    Synthetic Biology
    Tags
    ssrA
    Mutation
    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120225-plasmid-map-sequence-id-233264

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  18. pCas9CR4-VQR

    Plasmid
    #120224
    Purpose
    pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015
    Depositor
    Christopher Reisch
    Insert
    SpCas9-VQR
    Use
    Synthetic Biology
    Tags
    ssrA
    Mutation
    Mutations D1135V/R1335V/T1337R
    Promoter
    pTet
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120224-plasmid-map-sequence-id-233296

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  19. Phage-PGK-FLAG-HA-T(WT)

    Plasmid
    #181737
    Purpose
    for lentiviral expression of brachyury ORF in mammalian cells
    Depositor
    Charles Lin
    Insert
    T (brachyury)
    Use
    Lentiviral
    Mutation
    a silent mutation in the PAM site of an sgRNAt ta…
    Available Since
    Aug. 3, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    181737-plasmid-map-sequence-id-357225

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  20. hfCas12Max

    Plasmid
    #195336
    Purpose
    vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized hfCas12Max
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    N243R, E336R, D892R
    Promoter
    CBh, CMV, hU6
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    195336-plasmid-map-sequence-id-389250

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Showing: 681 - 700 of 2164 results