We narrowed to 3,960 results for: multiplex

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pYPQ155

Plasmid

#69306

Purpose

Gateway entry vector with AteCas9D10A

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9D10A (Arabidopsis codon optimized)

Use

CRISPR

Mutation

D10A mutation; NLS fusion

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x7

Plasmid

#58777

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLV floxed hMyoD-IRES-dsRed

Plasmid

#66632

Purpose

co-expresses CRE excisable human MyoD and dsRed

Depositor

Charles Gersbach

Insert

human MyoD IRES dsRed (MYOD1 Human)

Use

Cre/Lox and Lentiviral

Promoter

hUbC

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLibacceptorV2

Plasmid

#106250

Purpose

Recombination-mediated cassette exchange with target genomic loci

Depositor

Sriram Kosuri

Publication

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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BPK1169: CAG-DmrC-NLS-3xFLAG-P65

Plasmid

#104564

Purpose

Mammalian expression vector for DmrC fused to p65 activation domain

Depositor

Keith Joung

Publication

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

DmrC-p65

Tags

3x FLAG and NLS

Expression

Mammalian

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti-sgArid1a/Cre

Plasmid

#89642

Purpose

Expresses an Arid1a-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Publication

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Arid1a (Arid1a Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti_9xD3B_Cluc_Norm

Plasmid

#68414

Purpose

CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format.

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Cypridina Luciferase-2A-Venus YFP

Use

Lentiviral and Luciferase

Mutation

YFP: M153T,V163A,S175G ("Venus") YFP Va…

Promoter

minimalCMV

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pRec ΔLacI

Plasmid

#104575

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR constitutively.

Depositor

Harris Wang

Publication

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR

Use

Synthetic Biology

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x6

Plasmid

#58776

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pJSH-B14

Plasmid

#20145

Depositor

Glen Hartman

Publication

Haudenshield et al Plant Dis. 2011 Mar;95(3):343-352. doi:

Insert

Artificial sequence

Use

Cre/Lox

Mutation

n/a

Available since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNT3/Cre

Plasmid

#89654

Purpose

Expresses a non-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Publication

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

non-targeting gRNA

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV/MASC_(GLuc)_TOP1

Plasmid

#68439

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCRA03

Plasmid

#103141

Purpose

Triple gRNA expression separated with 28nt Csy4 motif

Depositor

Jens Nielsen

Publication

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

gRNAs

Expression

Yeast

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x5

Plasmid

#58775

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRb1/Cre

Plasmid

#89647

Purpose

Expresses an Rb1-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Publication

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Rb1 (Rb1 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ135A

Plasmid

#69277

Purpose

Golden Gate entry vector; 5th gRNA under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ141C

Plasmid

#69292

Purpose

Gateway entry vector; single gRNA under OsU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAC92-pmax-dCas9VP96

Plasmid

#48224

Purpose

dCas9VP96 on pmax expression vector

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP96 activation domain

Use

CRISPR

Tags

HA Tag and VP96

Expression

Mammalian

Mutation

D10A;H840A

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pYPQ136A

Plasmid

#69278

Purpose

Golden Gate entry vector; 6th gRNA under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ145

Plasmid

#69297

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 5 gRNAs

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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