We narrowed to 4,695 results for: pcr

1. pCRII-TOPO CMV-cGFP-mMEN b_3' Sense

   Plasmid

   #46840

   Purpose

   Express cGFP transcript ending in the wildtype MEN beta triple helix

   Depositor

   Phil Sharp

   Article

   Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

   Insert

   Coral Green Fluorescent Protein (cGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCru5-ACC/ACC/ACC-mEGFP-IRES-mCherry

   Plasmid

   #49219

   Purpose

   Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Retroviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Mutation

   Promoter

   Viral LTR (or CMV)

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRII-TOPO CMV-cGFP-mMALAT1_3' Mut U1/U2 Sense

   Plasmid

   #46837

   Purpose

   Express cGFP transcript ending in a mutant MALAT1 triple helix which causes the transcript to be degraded

   Depositor

   Phil Sharp

   Article

   Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

   Insert

   Coral Green Fluorescent Protein (cGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2

   Plasmid

   #91802

   Purpose

   Expresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be used

   Depositor

   Jeremy Wilusz

   Article

   Liang et al Methods. 2021 Apr 18. pii: S1046-2023(21

   Insert

   Coral Green Fluorescent Protein (cGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCR3-vsv-CenpB 1-166-dCEN-Incenp-GFP

   Plasmid

   #108486

   Purpose

   inducible expression of vsv-Mis12-dCEN-INCENP-EGFP

   Depositor

   Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   CenpB-dCEN-INCENP (INCENP Human)
   

   Use

   Tags

   EGFP and VSV

   Expression

   Mammalian

   Mutation

   CEN box replaced by CenpB 1-166

   Promoter

   CMV

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

   Plasmid

   #221235

   Purpose

   Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

   Plasmid

   #140198

   Purpose

   Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Insert

   dLbCas12a(D156R)-VPR
   

   Use

   CRISPR and Synthetic Biology; Expression in asper…

   Tags

   VPR (VP64-p65-Rta), NLS

   Expression

   Mutation

   D832A DNase deactivated, D156R for improved activ…

   Promoter

   PgpdA

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRII-TOPO CMV-ZKSCAN1 nt 400-1782-mMALAT1_3' Sense

   Plasmid

   #60647

   Purpose

   Expresses the human ZKSCAN1 exon 2/3 circular RNA

   Depositor

   Jeremy Wilusz

   Article

   Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

   Insert

   ZKSCAN1 (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

   Plasmid

   #140205

   Purpose

   Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Inserts

   mCherry
   sgRNA E1
   

   Use

   CRISPR and Synthetic Biology; Proof-of-concept te…

   Tags

   Expression

   Mutation

   G174D

   Promoter

   PelcA and U3

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRII-TOPO CMV-ZKSCAN1 nt 400-1782-bGH pA Sense

    Plasmid

    #60645

    Purpose

    Expresses the human ZKSCAN1 exon 2/3 circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid

    #49218

    Purpose

    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Retroviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    Viral LTR (or CMV)

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRII-TOPO CMV-ZKSCAN1 nt 400-1782-SV40 pA Sense

    Plasmid

    #60643

    Purpose

    Expresses the human ZKSCAN1 exon 2/3 circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

    Plasmid

    #110201

    Purpose

    Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    LexPR transactivator - 2A - Cerulean
    

    Use

    Tags

    Cerulean

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. tet-AID-PcrA M6-UGI-T2A-mTagBFP2-PGK-Puro-T2A-rtTA

    Plasmid

    #221234

    Purpose

    Tet-inducible lentiviral HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-PcrA M6-UGI-T2A-mTagBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCri System

    Plasmid Kit

    #1000000058

    Purpose

    pCri System: plasmids for cytoplasmic and periplasmic/extracellular heterologous protein expression in Escherichia coli, Bacillus subtilis, and Pichia pastoris.

    Depositor

    F Xavier Gomis-Ruth

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. Yeast GPCR-sensor Toolkit

    Plasmid Kit

    #1000000157

    Purpose

    The Yeast GPCR-sensor Toolkit includes 42 plasmids for constructing highly-tunable GPCR-based biosensors in yeast.

    Depositor

    Tom Ellis

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

17. pFA6a-A1:natMX4

    Plasmid

    #134428

    Purpose

    PCR-based C-terminal A1 tag

    Depositor

    Robert Tomko

    Article

    Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

    Insert

    A1
    

    Use

    Pcr-based tagging in yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pFA6a-SRS:hphMX4

    Plasmid

    #134441

    Purpose

    PCR-based C-terminal SRS tag

    Depositor

    Robert Tomko

    Article

    Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

    Insert

    SRS
    

    Use

    Pcr-based tagging in yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pFA6a-SRS:natMX4

    Plasmid

    #134444

    Purpose

    PCR-based C-terminal SRS tag

    Depositor

    Robert Tomko

    Article

    Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

    Insert

    SRS
    

    Use

    Pcr-based tagging in yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pFA6a-S6:natMX4

    Plasmid

    #134436

    Purpose

    PCR-based C-terminal S6 tag

    Depositor

    Robert Tomko

    Article

    Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

    Insert

    S6
    

    Use

    Pcr-based tagging in yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge