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Plasmid#219537Purposea. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotorDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
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pLAI-Env
Plasmid#133996PurposeEnv gene from HIV-1 LAI in pcDNA3.1 expression vectorDepositorInsertEnvelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNatB (pACYCduet-naa20-naa25)
Plasmid#53613PurposeAllows expression of the fission yeast NatB complex - chloramphenicol markerDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK015
Plasmid#105347PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK013
Plasmid#105345PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK016
Plasmid#105348PurposeBacterial translocation for proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK014
Plasmid#105346PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUD-MSP1D1
Plasmid#161608PurposeBacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formationDepositorInsertUD-MSP1D1
UseTags7-His tagExpressionBacterialMutationmutant of MSP1D1; all tryptophans and tyrosines w…PromoterT7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-VP40
Plasmid#74421PurposeMammalian expression of Ebolavirus matrix protein VP40 with an mCherry fusionDepositorInsertVP40
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
AIO-mCherry
Plasmid#74120PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNTI661 pRPR1(TetO)-sgRNA
Plasmid#139475PurposeVector for tet-regulated sgRNA expression in yeastDepositorInsertsingle guide RNA scaffold
UseCRISPRTagsExpressionYeastMutationPromoterpRPR1(TetO)Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TRAIL
Plasmid#10953DepositorInsertTumor necrosis factor alpha related apoptosis inducing ligand (TNFSF10 Human)
UseTagsGFPExpressionMammalianMutationNAPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
AIO-GFP
Plasmid#74119PurposeAll-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-nlsCRE
Plasmid#12265DepositorInsertCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
Spacer Switch Plasmid
Plasmid#215346PurposeFacilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.DepositorInsertOpaD spacer; Chloramphenicol Resistance Cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAJT194
Plasmid#202293PurposeType IIS cloning plasmid for LPattB5 integrations w/ OriTDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD-MSP1D1
Plasmid#161607PurposeBacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formationDepositorInsertD-MSP1D1
UseTags7-His tagExpressionBacterialMutationmutant of MSP1D1; all tryptophans were replaced b…PromoterT7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPfor
Plasmid#158059PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAUC40
Plasmid#71298PurposeSuicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLOX-CW-CRE
Plasmid#12238DepositorInsertCRE
UseCre/Lox and LentiviralTagsExpressionMammalianMutationCMV promoter is upstream of nls-CRE. LoxP in 3…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only