We narrowed to 188 results for: phen

...p-azido-l-phenylalanine Bacterial TAG Peter Schultz 31190 pEVOL-pBpF p-benzoylphenylalanine RS M. jannaschii...Tirrell 62598 pKPY514 phenylalanyl-tRNA synthetase subunit E. coli p-azido-L-phenylalanine (Azf) C. elegans... 62599 pKPY197 phenylalanyl-tRNA synthetase (CePheRS) C.elegans p-azido-L-phenylalanine (Azf) C. elegans...85494 pDule-pCNF para-cyanophenylalanine synthetase M. jannaschii azidoPhenylalanine Bacterial TAG Ryan ...85495 pDule2-pCNF para-cyanophenylalanine synthetase M. jannaschii azidoPhenylalanine Bacterial TAG Ryan ...jannaschii p-benzoyl-l-phenylalanine Bacterial TAG Peter Schultz 48215 pULTRA-CNF tyrosyl-tRNA synthetase...synthetase M. jannaschii para-cyanophenylalanine (pCNPhe) Bacterial TAG Peter Schultz 48696 pANAP AnapRS E. coli...

...variation affects genome function to influence phenotypes....affects genome function, which in turn impacts phenotype. The National Human Genome Research Institute ...characterize, and map how genome function shapes phenotype, and how these processes are affected by genomic...impact of genomic variants on genome function and phenotypes. Plasmids The collection will grow as the IGVF...impact of genomic variation on genome function and phenotype and shares their plasmid tools through Addgene...

...Chloride (Cl-) ClopHensor biosensor for intracellular chloride and pH Arosio Lab ClopHensor Plasmids Daniele...for intracellular chloride and pH (ClopHensor) Arosio Lab ClopHensor Plasmids Daniele Arosio Redox/ROS ...Daniele Arosio Chloride (Cl-) Dual ClopHensorN chloride and proton sensor A genetically-encoded chloride...:e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic) Multiplexed Optical...single-vesicle imaging. Neuron. 2009 Feb 12;61(3):397-411. Stephen Heinemann , Yongling Zhu Mitophagy Dual-emission...

...isogenic cellular background. All cell lines and phenotypic datasets that result from this effort will be...Figure 1: Schematic of iNDI iPSC line generation, phenotyping, and distribution efforts. Plasmids The following...

...TALEN inserts in one cloning step rather than two. Stephen Ekker Golden Gate TALEN 2.0 Golden Gate cloning...with Golden Gate TALEN and TAL Effector Kit 2.0. Stephen Ekker Platinum Gate TALEN Kit Platinum TALENs assembled...

...transduction. See plasmid 46948 for additional variant. Stephen Elledge 44011 pInducer10-mir-RUP-PheS 3rd Lentiviral...Meerbrey et al. (2011) for additional variants. Stephen Elledge 21373 pHIV-EGFP 3rd EF-1alpha driven expression...

...with dox- or tet-inducible luciferase expression Stephen Tapscott 83281 pAAV-CAG-FLuc Firefly CAG AAV expression...Red-shifted bioluminescence when paired with diphenylterazine (DTZ). LuxSit-i and neoLux1.2 : Artificial...

...targeted gene. However, the strength of the knockout phenotype for a given mutant cell must be validated experimentally... (often called hits) that result in a desired phenotype. The ease of gRNA design and synthesis, as well...to screen for novel genes that regulate known phenotypes, including resistances to chemotherapy drugs ...

...Santa Barbara Martin Schwartz , Yale University Stephen Sligar , University of Illinois Ron Vale , University...

...mCherry Parkinson's Stephen Tait 61728 YFP-Parkin-IRES-zeo PRKN YFP Parkinson's Stephen Tait 61856 MLM3636... DCTN1 GFP CMV ALS, motor neuron disease David Stephens 36382 pSMP-DNMT1_1 DNMT1 Hereditary sensory neuropathy...pcDNAFLAG-ATM-kd ATM Flag, His CMV Ataxia telangiectasia Stephen Elledge 43918 tetO-ALN NR4A2 His, V5 TRE Parkinson's...HIP1R-GFP-FKBP HIP1R GFP, FKBP CMV Parkinson's Stephen Royle 101223 pHR-FUSN-mCh-Cry2WT FUS SFFV ALS Clifford...

...residual guanidine thiocyanate. Phenol is a unique case, as pure phenol absorbs strongly at ~270 nm and...experiments. The usual suspects, such as proteins, phenol, or guanidine salts, exhibit their own characteristic...ones most likely to impact absorbance readings are phenol, guanidine salts, and detergents (Figure 1C-D; ...means that even samples with quite high levels of phenol (up to 600 ppm according to Loughrey and Matlock...yield “good” A260/280 and A260/230 ratios. Instead, phenol is more likely to shift the peak of your sample's...towards 270 nm. If your purification protocol uses phenol, then it is important to confirm that your sample...— if the peak is closer to 270 nm, you may have phenol in your sample.  RNA contaminants DNA samples can...

...Aug 20, 2020. What genes are important in your phenotype of interest? Many scientists study diseases for.... Identifying which genes are important for a phenotype can lead to a wealth of additional experiments...identify genes that are essential for a given phenotype. Image Credit: www.flickr.com/photos/[email protected] to identify genes involved in a particular phenotype. CRISPR can be readily scaled up for genome-wide...fluorescence-based cell sorting and screened for a particular phenotype. For example, mutant cells can be used in drug...particular gene is actually important for a given phenotype. Each hit from the screen should be independently...ensure that the desired modification produces the phenotype you screened for in the first place. With the ...

...mutant mice that display early ageing-associated phenotypes. Tyner SD, Venkatachalam S, Choi J, Jones S, ...

...effects. This conclusion was based upon numerous phenotype discrepancies between gene silencing and knockout...skipping, alternative start codons, and/or the phenomenon of transcriptional adaptation (Smits et al., ...delivering the RNAi trigger characterizing the RNAi phenotype. Although all these steps are areas of active...use and capacity to generate a long-term RNAi phenotype. Long hairpin RNA The easiest approach to induce...easily identified and have readily observable phenotypes quantified. Fluorescent reporter-based RNAi constructs... RNAi plants. With a visual reporter of RNAi, phenotypes, such as morphological parameters, can be easily...consuming, as RNAi mutants with a reduced growth phenotype will require additional plants to have enough ...

...acid-guanidinium-phenol solution that combines the homogenization solution and phenol addition of the ...extraction protocol with guanidinium thiocyanate-phenol-chloroform. In this method, samples are still homogenized...using cesium-chloride gradients, water-saturated phenol, sodium acetate, and chloroform are added to the...centrifugation (not ultracentrifugation!), the layers of phenol and chloroform separate, and RNA is retained in...of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction. Analytical Biochemistry ...of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on...

...genes you’d like to test for phenotypic effects. Typically the phenotype would be studied in vitro, but...growth properties, migration abilities, or other phenotypes that can be efficiently measured at the single... candidates for being involved in the studied phenotype. For robustness of a screen, each gene is not ...in a gene expression dataset are causal for a phenotype of interest. Selected pathways  I attended a great...With a custom CRISPR screen and the appropriate phenotypic readout, similar mapping could be rapidly performed...which signaling molecules were involved in the phenotype. Surface proteins  My group works on immune cells... a screen with an easily measureable cellular phenotype (e.g. survival of a cancer cell as a cytotoxic...

...between these two mice might drive their divergent phenotypes. Two labs, the Deverman lab at the Broad Institute... deletions (indels) most likely to have large phenotypic effects such as those that affected gene expression...genes were associated with the PHP.B permissive phenotype, but only one, Ly6a, showed differential protein... variant responsible for the PHP.B permissive phenotype in mice. They took a classic genetics approach...versions or alleles that was driving the PHP.B phenotype. Exome sequencing of related hybrid mice narrowed...candidate due to its strong linkage to the PHP.B phenotype and its expression pattern in the mouse brain....RNA expression patterns, the PHP.B permissive phenotype of mouse strains beside C57BL/6J and BALB/cJ, ...

... observed phenotype associated with the independent variable and helps ensure the phenotype isn’t singular...control plasmids help to ensure that the observed phenomenon in your experiment is specifically associated...plasmids should produce a null effect (i.e., no phenomenon is observed). In any plasmid-based experiment... control plasmids should produce the expected phenomenon. One example of a positive control, the internal...properly controlled experiments. The observed phenotype associated with the independent variable should...reproducibility or consistency of the observed phenotype associated with the independent variable. Biological...

...organisms) and then assaying for a functional or phenotypical outcome. In forward screens, there is random... population possessing a specific mutation or phenotype. For example, a drug screen may be performed on...enhance or suppress the previously defined disease phenotype. These screens employ the same downstream manipulations...the culture temperature to exacerbate a mutant phenotype, very similar to enhancer/suppressor screens. ...way, only cells or organisms with the desired phenotype will survive and be assayed at the end of the ...finish with the characterization of a function or phenotype in the surviving population. Selections do require...

...exciting computational algorithms, such as SIFT or PolyPhen to approach this challenge, using attributes of...molecular signatures of variant function and cancer phenotypes. Broad Institute Target Accelerator Plasmid ...measuring a variety of molecular signatures or cancer phenotypes. These assays were designed to complement the... cancer variants and genes: High-throughput Phenotyping of Lung Cancer Somatic Mutations. Berger AH, ...mutations and map other cellular functions and phenotypes. Since time is of the essence in clinical settings...recently demonstrated the power of this approach: Phenotypic Characterization of a Comprehensive Set of MAPK1...