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... Optional: Perform phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction...When phenol is mixed with the aqueous solution containing DNA, proteins will move into the phenol phase...Protocol: Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated phenol-chloroform... solution 2 mg/mL RNase A TE or water-saturated phenol-chloroform Chloroform 100% ethanol or isopropanol...the aqueous DNA sample. Pro-Tip Water-saturated phenol-chloroform can be used if TE-saturated is not available...recovered aqueous DNA layer. Repeat steps 2-4. Note: Phenol-chloroform is a hazardous waste - DO NOT pour down...purified without a kit it is advisable to perform a phenol/chloroform extraction of the supernatant after ...

...serves as a cushion for genome-containing virions. Phenol red is added to clearly distinguish the steps. ...iodixanol and 7 mL of 1X PBS-MK buffer and 30 μL of phenol red 40% iodixanol step: mix 6.7 mL of 60% iodixanol...iodixanol step: mix 10 mL of 60% iodixanol and 45 μL of phenol red Underlay each solution into a QuickSeal tube...are clearly distinguishable thanks to the use of phenol red. Image adapted from Zolotukhin, S., et al. ...

...using an all-in-one acid guanidinium thiocyanate-phenol solution such as TRIzol®. For the precipitation...QIAzol® (for Protocol Option #2) Water-saturated Phenol 2 M Sodium Acetate pH 4 Chloroform/Isoamyl alcohol...thoroughly by inversion. Add 1 mL water-saturated phenol, mix thoroughly by inversion. Add 0.2 mL of Chloroform...

...medium appears orange/yellow if using a standard phenol red pH indicator) because the buildup of waste ...the lines for transgene expression and/or other phenotypes. For example, perform Western blotting to screen...

...that target the same gene can produce the same phenotype will alleviate concerns about off-target effects...days Protein knockdown (western blot) ≥4 days Phenotypic assay ≥4 days Back to Top G. Safety BL2 safety...

...use Watch the Video! DNA Purification Miniprep, phenol-chloroform extract, and precipitate DNA DNA Quantification...

...mL Bleocin 5 µg/mL Carbenicillin 100 µg/mL Chloramphenicol 25 µg/mL Coumermycin 25 µg/mL Gentamycin 10...

...4% Paraformaldehyde 5 mg/mL 4′,6-diamidino-2-phenylindole (DAPI) Bovine serum albumin (BSA) Triton X-100...

...5 µg/mL Carbenicillin* 100 mg/mL 100 µg/mL Chloramphenicol 25 mg/mL (dissolve in EtOH) 25 µg/mL Coumermycin...

...VWR 45000-446 TMB (3,3',5,5'-tetramethyl-[1,1'-biphenyl]-4,4'-diamine) substrate kit, Pierce 34021 Tween...