Guide to Using Pooled Libraries
Type
Guide
...previously unknown genes that contribute to a phenotype. Pooled libraries consist of a single mixture ...understand what genes are important to a certain phenotype, and allow you to design additional hypothesis-directed...perturbations, and linking perturbations to single-cell phenotypes. These libraries are also commonly used to monitor... of mutant cells that are then screened for a phenotype of interest. Screening experiments using a pooled...technique that physically links a protein's function (phenotype) to the gene that encodes it (genotype). This ...chromatin accessibility Direct gain-of-function phenotypes Limitations Non-physiological expression Cannot...screening to elucidate which genes are involved in a phenotype. They are also useful for drug resistance screens...