We narrowed to 2,931 results for: prep
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Plasmid#228587PurposeExpresses OSCA1.2-K435L-L438K-eGFP in mammalian cellsDepositorInserthyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)
ExpressionMammalianMutationK435L, L438KAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGK Promoter (MTK 2) (pAN2822)
Plasmid#194224PurposeMammalian Toolkit part 2 encoding the PGK promoterDepositorInsertPGK Promoter
UseSynthetic BiologyPromoterPGKAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a Promoter (MTK 2) (pAN2824)
Plasmid#194225PurposeMammalian Toolkit part 2 containing the EF1a promoterDepositorInsertEF1a Promoter
UseSynthetic BiologyPromoterEF1aAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
LCB1_v1.3 (MTK 3a) (pZYW044)
Plasmid#194227PurposeMammalian Toolkit part 3a encoding the synthetic Spike binder LCB1DepositorInsertLCB1_v1.3
UseSynthetic BiologyPromoterNoneAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
LCB3_v1.2 (MTK 3a) (pZYW045)
Plasmid#194228PurposeMammalian Toolkit part 3a encoding the synthetic Spike binder LCB3DepositorInsertLCB3_v1.2
UseSynthetic BiologyPromoterNoneAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
WPRE (MTK 4b) (pAN2410)
Plasmid#194235PurposeMammalian Toolkit Part 4b encoding the WPRE for use with lentiviral backbonesDepositorInsertWPRE terminator
UseSynthetic BiologyPromoterNoneAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1302-AAV-EFSNC-dCjCas9-KRAB
Plasmid#223147PurposeExpression of KRAB with dCjCas9 and empty gRNA scaffoldDepositorInsertKRAB
UseAAV and CRISPRTags3xFLAGExpressionMammalianPromoterEF1aAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1312-AAV-EFSNC-dSaCas9-KRAB
Plasmid#223157PurposeExpression of KRAB with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB
UseAAV and CRISPRTags3xFLAGExpressionMammalianPromoterEF1aAvailable SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_HD_160-223AA
Plasmid#221489PurposeNKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationDeletion of 1-159AA N-terminal and 224-371AA C-te…PromoterEF1aAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
1114H
Plasmid#200640PurposeAn. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile malesDepositorInsertgRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
UseCRISPRExpressionInsectAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM5Z(+)-EGFP
Plasmid#65206PurposeEGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.DepositorInsertEGFP
ExpressionBacterial and MammalianMutationRestriction enzyme recognition sites added either…PromoterCMVAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
YY120: pPB_TRE::GEMINI(B)-p2A-GEMINI(A)
Plasmid#228881PurposePiggyBac plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a doxycycline-inducible promotor.DepositorInsertBuilding blocks of GEMINI for intracellular assembly
ExpressionMammalianPromoterTREAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE
Plasmid#223175PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR1_BsmBI
UseLentiviralMutationDR1 A>TAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
VPS13B^GFP
Plasmid#224588Purposecodon optimized GFP tagged VPS13B for expression in mammalian cellsDepositorAvailable SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOT_7-lenti-EFS-SS1-BBz-2A-puro
Plasmid#217990PurposeExpresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.DepositorInsertSS1-BBz CAR
UseLentiviralAvailable SinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-N-mEGFP-PARP7
Plasmid#178004PurposeExpresses N-terminally mEGFP-tagged PARP7DepositorAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Amber-free pNL4-3 FLuc Env-RT-
Plasmid#238415PurposeAn amber-free HIV NL4-3 carrying a Firefly luciferase reporter gene, RT and Env impaired.DepositorInsertFirefly luciferase (LOC116160065 Synthetic)
UseLentiviral and LuciferaseExpressionMammalianMutationAlternation of amber stop codons "TAG" …Available SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
EBOV 4cis
Plasmid#249332PurposeIntegration plasmid encoding EBOV essential replication factors (NP, VP35, VP30, and L protein)DepositorInsertEBOV NP-P2A-VP35-P2A-VP30-P2A-L protein
UsePiggybac donor vectorExpressionBacterial and MammalianMutationthe EBOV NP, VP35, and VP30 ORF sequences within …PromoterCAGAvailable SinceJan. 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
Plasmid#223173PurposeExpresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.DepositorInserthfCas13d (N2V8)
UseLentiviralTagsNLS-HAAvailable SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
YY229: pAAV_TRE::GFP- GEMINI(A)-P1N4_hSyn::rtTA3G
Plasmid#228889PurposeAAV plasmid expressing the destabilized GFP-GEMINI(A) under the control of a doxycycline-inducible promotor, and rtTA3G under the control of a hSyn promotorDepositorInsertGFP-GEMINI(A); rtTA3G
UseAAVTagsgfpPromoterTRE; hsynAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only