We narrowed to 11,132 results for: CHL

1. pAC-LYCipi

   Plasmid

   #53279

   Purpose

   Contains crtE, crtB, crtI, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces lycopene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

   Insert

   idi
   

   Use

   Low copy number bacterial cloning vector

   Promoter

   endogenous promoters

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAraH6HATT

   Plasmid

   #63901

   Purpose

   For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.

   Depositor

   Carlos Barbas

   Article

   Barbas et al Methods, A companion to Methods in Enzym

   Insert

   TT Fab
   

   Tags

   6xHis and HA tag

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVpuro-CMV-N-mCherry

   Plasmid

   #123221

   Purpose

   N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbS8c_ddCpf1_final

   Plasmid

   #212040

   Purpose

   Bacterial expression of the CRISPRi system for genome editing in E. coli

   Depositor

   Quanjiang Ji

   Article

   Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

   Insert

   ddCpf1
   

   Use

   CRISPR

   Promoter

   L-arabinose-inducible promoter PBAD

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST26-C-FLAG

   Plasmid

   #79275

   Purpose

   Destination vector containing a C-terminal FLAG tag

   Depositor

   Rita Shiang

   Article

   Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

   Insert

   FLAG
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 13, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pBA559

   Plasmid

   #186235

   Purpose

   pTet-LbuCas13a crRNA entry vector

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTH100

   Plasmid

   #84458

   Purpose

   pJB38-NWMN29-30 + SarA_P1-sGFP-Term

   Depositor

   Reindert Nijland

   Article

   de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

   Insert

   SarA_P1-sGFP from pCM29
   

   Tags

   6xHis (on sfGFP)

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLG010 (DRT type 2)

   Plasmid

   #157888

   Purpose

   Expresses the DRT type 2 defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   DRT type 2
   

   Expression

   Bacterial

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVpuro-CMV-N-3xFLAG

   Plasmid

   #123223

   Purpose

   N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKm-HCBE

    Plasmid

    #170333

    Purpose

    a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Insert

    Rhizobium codon-optimized CBEs
    

    Use

    CRISPR

    Mutation

    Cas9n-D10A

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNP1950

    Plasmid

    #222729

    Purpose

    J23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACYCDuet-1_Ec_rpoZ

    Plasmid

    #128837

    Purpose

    E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

    Depositor

    Seth Darst

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Insert

    rpoZ (rpoZ E. coli)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGWB-cLUC

    Plasmid

    #174051

    Purpose

    Gateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assay

    Depositor

    Alberto Macho

    Article

    Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Plant

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

    Plasmid

    #20298

    Purpose

    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    humanized ChR2 gene with histidine 134 changed to…

    Promoter

    EF1alpha

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGAMA-empty

    Plasmid

    #74755

    Purpose

    Mammalian lentiviral vector

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell Rep. 2016 Mar 15;14(10):2301-12. do

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWJ66

    Plasmid

    #46570

    Purpose

    Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    dcas9-w
    CRISPR array
    

    Use

    CRISPR

    Tags

    rpoZ

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB-nLUC

    Plasmid

    #174050

    Purpose

    Gateway compatible plasmid containing N-terminal luciferase for split-luciferase complementation assay

    Depositor

    Alberto Macho

    Article

    Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    N-terminal luciferase

    Expression

    Plant

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST-CMV-3xFLAG-gateway-EGFP

    Plasmid

    #122845

    Purpose

    N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBbS5c-mCherry-AsLOV2*(543)

    Plasmid

    #202712

    Purpose

    Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

    Depositor

    Mary Dunlop

    Article

    Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

    Insert

    mCherry-AsLOV2*(543)
    

    Expression

    Bacterial

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFZ19

    Plasmid

    #36184

    Depositor

    Daniel Voytas

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

    Type

    Empty backbone

    Use

    Plant t-dna vector

    Expression

    Plant

    Promoter

    XVE

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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