We narrowed to 7,126 results for: poly
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Plasmid#29182PurposeExpression of the eGFP reporter gene was detected (positive) in the brain, but not detected (negative) in the eye.DepositorAvailable SinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only
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MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE
Plasmid#131730PurposeMADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strainDepositorInsertTagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
UseCre/Lox and Synthetic Biology; Mosaic analysis fo…TagsTagBFP2-V5 tagExpressionMammalianMutationG12VPromoternone (4x polyA to mitigate episomal expression)Available SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMS1385
Plasmid#29184PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).DepositorAvailable SinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1383
Plasmid#29183PurposeExpression of the eGFP reporter gene was not detected in the brain using eGFP. Untested in the eye.DepositorAvailable SinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1102
Plasmid#29033PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748)DepositorInsertPle183 (S100B Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-Cre-NLSAvailable SinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1103
Plasmid#29034PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle184 (S100B Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-Cre-NLSAvailable SinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
FNL Combinatorial Cloning Platform
Plasmid Kit#1000000211PurposeA Frederick National Laboratory (FNL) combinatorial cloning set to generate many tagged expression clones in parallel for protein production, in vivo gene expression, and shRNA and CRISPR delivery.DepositorApplicationCloning and Synthetic Biology, Gene Expression and LabelingVector TypeBacterial Expression, Insect Expression, Mammalian ExpressionCloning TypeMultiSite GatewayAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-GBX2-CDX124-Ef1a-mCherry-Neo
Plasmid#192823PurposeExpresses mCherry and four sgRNAs: GBX2, CDX1, CDX2, CDX4DepositorUseCRISPR and Cre/LoxExpressionMammalianAvailable SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7
Plasmid#214140PurposeInsect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7DepositorInsertsTags2xSTIIExpressionInsectPromoterpolyhedrinAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_RFWD3:425-774
Plasmid#210884PurposeInsect expression of human RFWD3 fragment S425 to E774. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertRFWD3:S425-E774 (RFWD3 Human)
TagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_SEC31A:1-338
Plasmid#210925PurposeInsect expression of human SEC31A fragment M1 to T338. N-terminal 6X His tag with TEV cleavage.DepositorInsertSEC31A:M1-T338 (SEC31A Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-LIC_DDA1:1-102
Plasmid#210902PurposeInsect expression of full-length human DDA1. Untagged.DepositorAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_RACK1:1-317
Plasmid#210889PurposeInsect expression of full-length human RACK1. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertRACK1:M1-R317 (RACK1 Human)
TagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII
Plasmid#214139PurposeInsect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2DepositorInsertsTags2xSTIIExpressionInsectPromoterpolyhedrinAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-LIC_SMU1:1-513
Plasmid#210877PurposeInsect expression of full-length human SMU1. N-terminal 6X His tag with thrombin protease cleavage site.DepositorInsertSMU1:M1-P513 (SMU1 Human)
TagsMGSSHHHHHHSSGLVPRGSExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBiT3.1-N_DCAF8L2:1-631
Plasmid#211239PurposeMammalian expression of full-length human DCAF8L2. N-terminal HiBiT tag.DepositorInsertDCAF8L2:M1-S631 (DCAF8L2 Human)
TagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutation145-147: missing in Poly E regionPromoterCMVAvailable SinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-LIC_MAGEA3:104-314
Plasmid#210941PurposeInsect expression of human MAGEA3 fragment E104 to E314. Untagged.DepositorAvailable SinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_UTP15:1-370
Plasmid#210937PurposeInsect expression of human UTP15 fragment M1 to H370. N-terminal 6X His tag with TEV cleavage.DepositorInsertUTP15:M1-H370 (UTP15 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_TBL2:70-405
Plasmid#210936PurposeInsect expression of human TBL2 fragment E70 to H405. N-terminal 6X His tag with TEV cleavage.DepositorInsertTBL2:E70-H405 (TBL2 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL_SHKBP1:182-585
Plasmid#210935PurposeInsect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.DepositorInsertSHKBP1:T182-L585 (SHKBP1 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationQ507L natural variant (VAR_036714)PromoterPolyhedrinAvailable SinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits