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pCAG-GW-V5-Hygro

Plasmid

#68265

Purpose

Constitutive selectable expression of C-term V5 tagged proteins

Depositor

Mitchell Guttman

Article

McHugh et al Nature. 2015 May 14;521(7551):232-6. doi

Type

Empty backbone

Tags

V5

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only
bdSUMO-R9-GFP-HiBit

Plasmid

#170990

Purpose

For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-R9-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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px330-RPL26 sgRNA2

Plasmid

#134642

Purpose

contains sgRNA targeting to the C-terminus of human RPL26 for gene editing

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

RPL26 sgRNA2 (RPL26 Human)

Expression

Mammalian

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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px330-RPL26 sgRNA1

Plasmid

#134641

Purpose

contains sgRNA targeting to the C-terminus of human RPL26 for gene editing

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

RPL26 sgRNA2 (RPL26 Human)

Expression

Mammalian

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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px330-UFSP2 sgRNA1

Plasmid

#134638

Purpose

contains sgRNA targeting human UFSP2 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFSP2 sgRNA1 (UFSP2 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pYO539

Plasmid

#235752

Purpose

Protein expression of ScVPS30 and ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Expression

Yeast

Promoter

GAL-TDH3

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF5)-TS.FF5x1-bGH

Plasmid

#235277

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mGL-bGH

Plasmid

#235299

Purpose

mGreenLantern expression to serve as a transfection marker, EF1a-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF4)-TS.FF6x1-bGH

Plasmid

#235282

Purpose

ComMAND open-loop circuit regulating mRuby2 with miR-FF4

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF5)-TS.FF6x1-bGH

Plasmid

#235260

Purpose

ComMAND open-loop circuit regulating mRuby2 with miRE-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-mGL-bGH

Plasmid

#235255

Purpose

mGreenLantern expression to serve as a transfection marker, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pYO734

Plasmid

#235753

Purpose

Protein expression of ScVPS30 and ZZ-ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Tags

ZZ-3xTEV

Expression

Yeast

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF5)-TS.FF6x1-bGH

Plasmid

#235276

Purpose

ComMAND open-loop circuit regulating mRuby2 with miR-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235270

Purpose

ComMAND open-loop circuit regulating mRuby2, EF1a

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235271

Purpose

ComMAND closed-loop circuit regulating mRuby2, EF1a

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF4)-TS.FF4x1-bGH

Plasmid

#235283

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF4

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TS.FF4x1-mRuby2-bGH

Plasmid

#235274

Purpose

3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF5)-TS.FF5x1-bGH

Plasmid

#235261

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miRE-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-tagBFP-bGH

Plasmid

#235257

Purpose

tagBFP expression to serve as a "filler" plasmid for transfections

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

tagBFP

Use

Synthetic Biology

Expression

Mammalian

Mutation

Silent mutations to remove BsaI sites in tagBFP

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-mRuby2-bGH

Plasmid

#235253

Purpose

ComMAND base gene regulating mRuby2, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge