We narrowed to 13,494 results for: Mpl;

1. qTAG-C-mNeon-Blast-TUBB4B

   Plasmid

   #207786

   Purpose

   Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJZC32

   Plasmid

   #62327

   Purpose

   sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA
   MCP-VP64
   

   Use

   Lentiviral

   Tags

   VP64

   Expression

   Mammalian

   Mutation

   Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

   Promoter

   CMV and U6

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEBTet-SNAP-Cep57

   Plasmid

   #136817

   Purpose

   Mammalian expression of the centrosomal protein Cep57 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-Cep57 (CEP57 Human, Synthetic)
   

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Promoter

   CMV-TetO2

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. gp64-mVEGF-C-HisTag-pENTR

   Plasmid

   #64065

   Purpose

   Plasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

   Insert

   vascular endothelial growth factor C (Vegfc Mouse)
   

   Tags

   His-tag

   Expression

   Insect

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pODN-AAVS1-mR2-CAAX

   Plasmid

   #183871

   Purpose

   Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mRuby2-CAAX

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   CMV

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIneo-lambdaN-HA-HsDCP1a_G

   Plasmid

   #146400

   Purpose

   Mammalian Expression of HsDcp1a

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   HsDcp1a (DCP1A Human)
   

   Expression

   Mammalian

   Mutation

   two silent mutations T237C and G1716T compared to…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA4.TO-ORF75-2xCSTREP

   Plasmid

   #136234

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF75

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF75
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LN-entry+NLS

   Plasmid

   #128011

   Purpose

   Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   LN-3xFlag-SV40NLS
   

   Use

   Lamdan entry vector with nls to generate tetherin…

   Tags

   LN-3xFlag-SV40NLS

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST27-GST-ATXN1 FL(30Q)

   Plasmid

   #21753

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 32 amino acids (30 Q…

   Promoter

   CMV

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

10. myc-Atg14 S29D

    Plasmid

    #86757

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S29D

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. HOXD4 (murine) MSCV

    Plasmid

    #8549

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXD4 (Hoxd4 Mouse)
    

    Use

    Retroviral

    Tags

    His and T7

    Expression

    Mammalian

    Mutation

    contains T7 and HIS tags from pET; makes protein …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

12. HA-VHL C162F-pRc/CMV

    Plasmid

    #22042

    Depositor

    William Kaelin

    Article

    Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    point mutant, converting aa 162 from Cysteine to …

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG GST DEPDC5

    Plasmid

    #164983

    Purpose

    DEPDC5 expressing vector for mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    DEPDC5 (DEPDC5 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. myc-Atg14 S29A

    Plasmid

    #86756

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pnEA-NpM-HsNot4-GB1His6_AG

    Plasmid

    #148792

    Purpose

    Bacterial Expression of HsNot4

    Depositor

    Elisa Izaurralde

    Article

    Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

    Insert

    HsNot4 (CNOT4 Human)
    

    Expression

    Bacterial

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST47-CYFIP2.GFP

    Plasmid

    #109140

    Purpose

    GFP-tagged CYFIP2 expression construct

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP2 (CYFIP2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG8H-EWS_1-264

    Plasmid

    #180467

    Purpose

    Expresses the construct His8-EWS_1-264 with a TEV cleavage site for the removal of the His8 tag

    Depositor

    David Libich

    Article

    Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

    Insert

    EWSR1 (EWSR1 Human)
    

    Tags

    His8

    Expression

    Bacterial

    Mutation

    deleted 265-656

    Promoter

    T7

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA4.TO-K8b-2xCSTREP

    Plasmid

    #136159

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K8b

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    K8b
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA4.TO-ORF25-2xCSTREP

    Plasmid

    #136185

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF25

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF25
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA4.TO-ORF53-2xCSTREP

    Plasmid

    #136213

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF53

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF53
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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