We narrowed to 13,650 results for: Mpl;

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pcDNA5-FRT-TO-FH-Nsp10

Plasmid

#157707

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp10 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR4-EGFP_opt

Plasmid

#106334

Purpose

Gateway Entry vector for EGFP (codon optimized for efficient gene synthesis)

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

EGFP

Use

Gateway entry vector

Expression

Bacterial and Mammalian

Mutation

codon optimized for gene synthesis without affect…

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K2-2xCSTREP

Plasmid

#136149

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K2

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pMT1-24X-NOG(CmYLCV)

Plasmid

#202014

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

dCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1

Tags

GB1 and sfGFP

Expression

Plant

Promoter

CmYLCV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-EGFP-T2A-RPL22-1xFlag

Plasmid

#170323

Purpose

Expresses FLAG and EGFP tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Cep164

Plasmid

#136873

Purpose

Mammalian expression of the centrosomal protein Cep164 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep164 (CEP164 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-FAM208A ts1

Plasmid

#115871

Purpose

FAM208a knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

FAM208a shRNA (C3orf63 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF65-2xCSTREP

Plasmid

#136225

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF65

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF65

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK-Hygro-Myc-hAgo2

Plasmid

#60234

Purpose

Express HA-tagged wild-type Ago2 in mammalian cells in a Dox-inducible manner

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2012 May 25;46(4):507-17. doi:

Insert

Argonaute2 (AGO2 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-Nprot

Plasmid

#157728

Purpose

mammalian expression of untagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nprot (N SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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FpG5.BFP.Reporter

Plasmid

#134967

Purpose

Lentiviral vector for enhancer reporter assay

Depositor

Beat Bornhauser

Article

Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

mini Fgf4 promoter

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE hygro MEN1 A242V

Plasmid

#11022

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 A242V (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

A242V

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pJZC48

Plasmid

#62336

Purpose

sgRNA + 1x COM with COM-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 1x COM binding module
COM-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-C9ORF80

Plasmid

#128418

Purpose

Express C9ORF80 in E. coli with a His tag (C-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

C9ORF80 (INIP Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp14-HF

Plasmid

#157719

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp14-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTE2690

Plasmid

#137717

Purpose

Expresses dimpLbCas12a-SunTag in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

Insert

dimpLbCas12a

Use

CRISPR

Tags

NLSx3 SV40 and SunTagx10

Expression

Mammalian

Mutation

D156R, G532R, K538V, Y542R, K595R, D832A,

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp12

Plasmid

#157710

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp12 (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pF148 pSOD1G37RAcGFP1

Plasmid

#26409

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

G37R

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only
pTYB11-PTBP1-RRM1

Plasmid

#89153

Purpose

Recombinant expression of PTBP1-RRM1 in E.coli

Depositor

Frédéric Allain

Article

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1 (PTBP1 Human)

Tags

Intein and chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-mCherry-T2A-RPL22-1xV5

Plasmid

#170324

Purpose

Expresses V5 and mCherry tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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