We narrowed to 8,588 results for: Dos
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Plasmid#192466PurposeBacterial expression of RvCAHS3DepositorInsertCAHS3
TagsHis6ExpressionBacterialPromoterT7Available sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-Cenexin-S796A
Plasmid#186434PurposeExpresses mCherry Cenexin with S796A mutation in mammalian cells and zebrafish embryosDepositorInsertCenexin (ODF2 Human)
TagsmCherryExpressionMammalianMutationChanged Serine 796 to AlaninePromoterCMV IE94Available sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB345
Plasmid#185078Purposerfa3D46 truncation fused to GFPDepositorInsertRFA3
TagsGFPExpressionYeastMutationRfa3 truncation aa 1-46Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB349
Plasmid#185080Purposerfa2D248 truncation fused to GFPDepositorInsertRFA2
TagsGFPExpressionYeastMutationRfa2 truncation aa 1-247Available sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hPPP3R2
Plasmid#179163PurposeExpression vector of human protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2), CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2 Human)
ExpressionMammalianAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mPpp3r2
Plasmid#179161PurposeExpression vector of mouse protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2), CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2 Mouse)
ExpressionMammalianAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-43
Plasmid#172759PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-43; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-43
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-35
Plasmid#172755PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-35; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-35
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-19
Plasmid#172748PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-19; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-19
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-3
Plasmid#172740PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-3
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-12
Plasmid#172744PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-12
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-14
Plasmid#172745PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-14
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-4
Plasmid#163142PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-3
Plasmid#163141PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-2
Plasmid#163140PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG438
Plasmid#165486PurposeVector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAGDepositorInsertMammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
UseCRISPRTags3x FLAG and SV40 NLSExpressionMammalianMutationD1135V, S1136R, D1332K, and R1333G mutations in S…PromoterCMV and T7Available sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG442
Plasmid#165616PurposeVector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)DepositorInsertTEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
UseCRISPRTags3x HA, SV40 NLS, Zif268, and c-Myc-like NLSExpressionYeastMutationCorrected homology relative to wild type SpCas9 (…PromoterTEF1Available sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1HR1AtR2NtB2
Plasmid#160890PurposeExpression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-S1H
Tags6xHisExpressionBacterialPromoterrbcS-SH1: T7, chaperones: T13Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtT4aR1AtR2NtB2
Plasmid#160887PurposeExpression of N. tabacum Rubisco small subunit (rbcS-T4a), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-T4a
ExpressionBacterialPromoterrbcS-T4a: T7, chaperones: T10Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL052
Plasmid#137906PurposeOsBiP1 promoter driven Cas9-tRNA systemDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only