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pMuLE ENTR 7SK-miR-30 L1-R5

Plasmid

#62118

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Fhod1-3'UTR-MRE-2 MUT

Plasmid

#61787

Purpose

Luciferase reporter containing the 3'UTR of mouse Fhod1 with MRE-2 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Fhod1 3'UTR with miR-200c MRE-2 mutated

Use

Luciferase

Mutation

miR-200c MRE-2 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Tob1-3'UTR-MRE-1 MUT

Plasmid

#61788

Purpose

Luciferase reporter containing the 3'UTR of mouse Tob1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Tob1 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Zfp36-3'UTR-MRE-1 MUT

Plasmid

#61794

Purpose

Luciferase reporter containing the 3'UTR of mouse Zfp36 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Zfp36 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Snail1-3'UTR-MRE-1 MUT

Plasmid

#61795

Purpose

Luciferase reporter containing the 3'UTR of mouse Snail1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Snail1 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Mapk12-3'UTR-MRE-1 MUT

Plasmid

#61797

Purpose

Luciferase reporter containing the 3'UTR of mouse Mapk12 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Mapk12 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Alpha-0 Dronpa

Plasmid

#44193

Depositor

Narasimhan Gautam

Article

Chisari et al J Biol Chem. 2007 Aug 17;282(33):24092-8

Insert

G protein alpha 0 (Gnao1 Rat)

Tags

Dronpa

Expression

Mammalian

Mutation

Introduced KpnI and SpeI sites between 92nd and 9…

Promoter

CMV

Available Since

April 14, 2015

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR CMV iRFP R4-R3

Plasmid

#62158

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and iRFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

iRFP

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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TM9-bio-His

Plasmid

#51831

Purpose

Expresses full-length Transmembrane 9 superfamily member 4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

TM9 (TM9SF4 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 eGFP L5-L2

Plasmid

#62144

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and EGFP module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

eGFP

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

SV40

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-mIFITM3-Y20,27A

Plasmid

#58465

Purpose

Expresses murine IFITM3-Y20,27A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosines 20 and 27 to alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-mIFITM3-Y27A

Plasmid

#58464

Purpose

Expresses murine IFITM3-Y27A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 27 to alanine

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-H136T

Plasmid

#58413

Purpose

Expresses murine IFITM3-H136T with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Histidine 236 to Threonine

Promoter

CMV IE

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pMM2-hPMCA4bct120

Plasmid

#47760

Purpose

mammalian expression of human PMCA4 with C-terminal 120aa deletion

Depositor

John Penniston, Emanuel Strehler

Article

Enyedi et al J Biol Chem. 1993 May 15;268(14):10621-6

Insert

PMCA4bct120 (ATP2B4 Human)

Expression

Mammalian

Mutation

deletion of C-terminal 120 aa (removes calmodulin…

Promoter

SV40

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only
pPICZ:hTRAAK(W262S)

Plasmid

#130670

Purpose

P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

KCNK4 (KCNK4 Human)

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

N104Q, N108Q, W262S

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RNMT 1-120

Plasmid

#112706

Purpose

Expresses N-terminally FLAG-tagged human RNMT 1-120 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Tags

FLAG

Expression

Mammalian

Mutation

deleted amino acids 121-476

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RNMT 121-476

Plasmid

#112707

Purpose

Expresses N-terminally FLAG-tagged human RNMT 121-476 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Tags

FLAG

Expression

Mammalian

Mutation

deleted amino acids 2-120

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pIRES:hTRAAK(W262S)

Plasmid

#133078

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pIRES:hTRAAK(G124I)

Plasmid

#133079

Purpose

Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)

Expression

Mammalian

Mutation

G124I

Availability

Academic Institutions and Nonprofits only

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pSGDlucV3.0

Plasmid

#119760

Purpose

dual luciferase with FMDV StopGo sequences flanking MCS

Depositor

John Atkins

Article

Loughran et al Mol Cell. 2022 Oct 6;82(19):3745-3749.e2

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Promoter

SV40 and T7

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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