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Nav1.8 Na+ channel scFv [N134/12]

Plasmid

#190530

Purpose

Mammalian Expression of Nav1.8 Na+ channel scFV. Derived from hybridoma N134/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Nav1.8 Na+ channel (Rattus norvegicus) recombinant scFV (Scn10a Mouse)

Tags

Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Pan-Neurofascin (extracellular) scFv [A12/18]

Plasmid

#190485

Purpose

Mammalian Expression of Pan-Neurofascin (extracellular) scFV. Derived from hybridoma A12/18.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant scFV (Nfasc Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-tdTEVDB_C-15A/G4C

Plasmid

#177205

Purpose

Permuted td intron with evolved initiation sequence for the expression of circular mRNA

Depositor

Stephen Fried

Article

Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175

Insert

Permuted GFP with evolved initiation sequence for circular mRNA

Tags

6x His

Expression

Bacterial

Mutation

Permuted sfGFP after residue 52 & Changed Cyt…

Promoter

araBAD

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMEH305 HA NLS SpCas9 3x high affinity MS2 loops

Plasmid

#168216

Purpose

Mammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

SV40 NLS SpCas9

Use

Lentiviral

Tags

HA, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-E6AP Hect

Plasmid

#165102

Purpose

bacterial expression of a GST fusion of Human E6AP Hect domain

Depositor

Arthur Haas

Article

Ronchi et al Methods Mol Biol. 2012;832:197-218. doi:

Insert

E6AP Hect (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

E6AP Hect domain Amino acids 495-738 (5'QLNP…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRG714_lexO-AscI_LexA-TF_URA3MX

Plasmid

#154822

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3/flag-pBirA(1-G78)-FRB

Plasmid

#154447

Purpose

expresses pBirA(1-G78)-FRB/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

flag-pBirA(1-G78)-FRB

Expression

Mammalian

Promoter

T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pIE-hα2M-H6

Plasmid

#128492

Purpose

Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (A2M Human)

Tags

His tag (6x)

Expression

Insect

Promoter

IE1

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS

Plasmid

#123188

Purpose

binary plant vector for transient fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3A G120A

Plasmid

#123090

Purpose

Expresses 3xFLAG-LC3A G120A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 120 to Alanine

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-intron-BaLgp160

Plasmid

#100918

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env with upstream intron

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-BaLgp140-TM

Plasmid

#111843

Purpose

Mammalian expression plasmid for extracellular gp140 from the BaL HIV-1 strain fused to a transmembrane tether

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader sequence and TM helix from MHC class I

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPR_RAF1_94

Plasmid

#111826

Purpose

Knockout Raf1

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting RAF1 94-114 (RAF1 Human)

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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psbA2(noATbox)-PHLS (c)

Plasmid

#52308

Purpose

Replaces the psbA2 gene in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Mutation

CAAATACA box in the psbA2 promoter to ggcgcgcc

Promoter

psbA2

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAR3040-spitz

Plasmid

#38341

Depositor

Norbert Perrimon

Article

Rutledge et al Genes Dev. 1992 Aug;6(8):1503-17.

Insert

spitz (spi Fly)

Expression

Bacterial

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pCDF1-PTPRD-P1690F

Plasmid

#32525

Depositor

Todd Waldman

Article

Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

Insert

Protein tyrosine phosphatase receptor-type delta P1690F (PTPRD Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P1690F

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-PTPRD-G1707R

Plasmid

#32526

Depositor

Todd Waldman

Article

Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

Insert

Protein tyrosine phosphatase receptor-type delta G1707R (PTPRD Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G1707R

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
EC.SurA.TYB1.a172-274.wt

Plasmid

#15934

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

Peptidyl prolyl isomerase domain 1 of SurA (surA E. coli)

Tags

intein

Expression

Bacterial

Mutation

wild type

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pBABE-zeo small T 110

Plasmid

#9063

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 110 (SV40gp7 simian virus)

Use

Retroviral

Expression

Mammalian

Mutation

Contains only the N-termial 110 amino acids

Available Since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
pAAV_GR-RE_MLPmin_BC1092-luc2

Plasmid

#227112

Purpose

Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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