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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,083 results for: pET

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Showing: 7081 - 7100 of 10083 results

  2. MPRA_minCMV+GFP

    Plasmid
    #204866
    Purpose
    Vector containing GFP driven by minimal CMV promoter. This cassette can be cloned into MPRA vectors to serve as a reporter gene.
    Depositor
    Philipp Maass
    Publication
    Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    minCMV:GFP
    Expression
    Bacterial and Mammalian
    Promoter
    minimal CMV
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204866-plasmid-map-sequence-id-406381

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  3. His-DERA

    Plasmid
    #203225
    Purpose
    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Publication
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    deoxyribose aldolase
    Tags
    N-terminal TEV cleavable 6xHis tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203225-plasmid-map-sequence-id-400074

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  5. 5VC9 (CXXC4)

    Plasmid
    #101263
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CXXC4 (CXXC4 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101263-plasmid-map-sequence-id-197652

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  8. Fast-GCaMP-EF16

    Plasmid
    #52642
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Publication
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fast-GCaMP-EF16
    Expression
    Mammalian
    Mutation
    D431N/D433N/D435N/E442A mutated from GCaMP3
    Promoter
    T7
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. MUM1

    Plasmid
    #28273
    Depositor
    Cheryl Arrowsmith
    Insert
    MUM1 (MUM1 Human)
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains PWWP Domain (residues 405-538).
    Available since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. HECTD1

    Plasmid
    #32860
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HECTD1 (HECTD1 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 1879-1966 only
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. OTU04

    Plasmid
    #28157
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    OTU domain containing 7A (OTUD7A Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    UBA-like domain only
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. USP08 (2A9U)

    Plasmid
    #25224
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    usp08.hs.0001-0142 (USP8 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Dimerization Domain, contains amino acids 8-138
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pTCF176 HLA-A*02:01 (CD8 mut)

    Plasmid
    #180459
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*02:01 (CD8 mut)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    D227K, T228A, replacement of transmembrane domain…
    Available since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    180459-plasmid-map-sequence-id-355125

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  18. mOtop2_pGEMHE

    Plasmid
    #114675
    Purpose
    This construct was used to express mouse Otop2 in Xenopus laevis oocytes
    Depositor
    Emily Liman
    Publication
    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Otopetrin 2 (Otop2 Mouse)
    Use
    For generating the mrna, which can highly express…
    Promoter
    T7
    Available since
    July 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    114675-plasmid-map-sequence-id-221626

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  19. His 3C MKK3 1-347

    Plasmid
    #87878
    Purpose
    E. coli expression of MKK3 aa 1-347
    Depositor
    Peter Mace
    Insert
    MKK3 (MAP2K3 Human)
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 1-347
    Promoter
    T7
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87878-plasmid-map-sequence-id-174923

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  20. EPB41

    Plasmid
    #32040
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EPB41 (EPB41 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    FERM domain only (amino acids 1-279)
    Available since
    Jan. 13, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 7081 - 7100 of 10083 results